Screening Of Protein Markers For Down Syndrome Fetal And Identification Of The Protein Markers In Maternal Plasma

Background:Down's Syndrome(DS)is the most common disease in children with birth-defects caused by euchromosome aberration in the newborn,incidence of DS is very hig h,and patients are often characterized by congenital mental retardation,special face,body developmental delay,and often associated with congenital heart disease,low immunity and leukemia et al.The DS patients often cannot take care of themselves,and their social adaptation and labor ability are poor,They have brought a heavy burden for their families and society.There is no effective treatment for the disease,mainly depends on the prenatal diagn osis and prenatal screening to reduce the birth of children with down's syndrome.Howev er the traditional prenatal screening have high miss rate and high false positive.Prenatal diagnosis,such as amniotic cavity puncture,velamentum bombycinum sampling and umbi lical cord blood for fetal cells to cultivate and then karyotype analysis is the gold standa rd for prenatal diagnosis of down's syndrome.But these traumatic methods may lead to complications such as bleeding,infection and abortion.Therefore,it is extremely urgent to look for new biomarkers,so as to develop a non-invasive,high detection rate of prena tal screening method for Down syndrome baby.Purpose:using Western Blot technique to validate proteins expressed differentially betw een DS fetal umbilical cord blood plasma and health fetal umbilical cord blood plasma i n the peripheral blood plasma from pregnant women,so as to get new biomarkers for down syndrome(DS)of prenatal diagnostic,and establish a new method prenatal diagnos is of non-invasive.Methods:Taking umbilical cord blood by puncturing umbilical cord,and then make a def inite diagnosis for the women,who went to the Shenzhen people's hospital and the Chin ese people's liberation army 181st hospital for prenatal screening and be regarded for down syndrome suspected or high-risk pregnant women during April 2013 and January 2013,diagnosis by G banding karyotype analysis and FISH.Collected maternal peripheral blood(8 with DS fetal and 8 with normal fetal)from April 2013 to January 2014,and separated plasma.Combining with the characteristics of clinic,make proteins expressed differentially between DS fetal umbilical cord blood plasma and health fetal umbilical c ord blood plasma as the specific protein marker candidates and prepared monoclonal anti bodies,then used Western Blot technique to analyze for candidate markers.Results:In the maternal plasma of DS fetal,the Apolipoprotein E,ERO1-like protein alp ha,Serum amyloid P-component,Complement factor B,2-oxoglutarate dehydrogenase-like,Nucleosome assembly protein 1-like 1,Thymosin beta-10 were up regular proteins compar ed to the healthy control group,the DS group has a higher expression,which is agreed in the DS fetal umbilical cord blood plasma.Conclusion:The proteins Apolipoprotein E,ERO1-like protein alpha,Serum amyloid P-com ponent,Complement factor B,2-oxoglutarate dehydrogenase-like,Nucleosome assembly prot ein 1-like 1,Thymosin beta-10,all are up-regulation,all of them have potential to be pr enatal diagnosis biomarkers for down's syndrome.And these biomarkers may by can furt her reveal the pathogenesis of down's syndrome.