Active Constituents Of Paulownia Elongata Flower

Paulowniaelongata,a member of the plant family Paulowniaceae and a rich source of biologically active secondary metabolites,is traditionally used in Chinese herbal medicine.It is flower has a remarkble biologie activity,such as oxidation,resistance,antibaeterium and anti-inflammatory.This thesis is divided into three parts to show a systermatic study of the Paulownia elongata flower.1.Column chromatography and recrystallization were used for separation,NMRand MS spectroscopic methods were used for structure identification.12 compounds were isolated from Paulownia elongata flower,and respectively identified as:6-prenyl-3'-O-methyeriodyctiol(1),mimulone(2),diplacone(3),3'-O-methyldiplacone(4),3'-O-methyl-5'-hydroxydiplacone(5),3'-O-methyl-5'-O-methyl-diplacone(6),Diplacol(7),3'-O-methyldiplacol(8),3'-O-methyl-5'-methoxydiplacol(9),apigenin(10),hydroquinone(11),ursolic acid(12).The compounds 1-9 and 11 are isolated from Paulownia elongata for the first time,and compound 1 is isolated from Paulowniaceae for the first time.2.Using Vc and BHT as positive controls,anti-radical ability of components from Paulownia elongata was determined by DPPH method.The antioxidantion of the ten compounds size in the order:Diplacol(7)>diplacone(3)>hydroquinone(11)>3'-O-methyl-5'-hydroxydiplacone(5)>3'-O-methyl-diplacol(8)>6-preny 1-3'-O-methyeriodyctiol(1)>3'-O-methyldiplacone(4)>mimulone(2)>3'-O-methyl-5'?methoxy-diplacol(9)>apigenin(10).It shows that the antioxidantion is not only related to the the number and location of hydroxyl groups,but also the proportion of phenolic hydroxyl groups of compound molecular weight.3.The cytotoxicity of compounds was determined by MTT method.Compound diplacone(3)?3'-0-methy 1-5'-hydroxydiplacone(5)and apigenin(10)exhibited cytotoxicity against A-549,while compounds(5)shows cytotoxicity againstHela as well.