Study On Quality Standards Of Qiqing Baidu Powder

Objective:Entrusted by Inner Mongolia Runlong Biotechnology Co.,Ltd.,the quality standard of Qiqing baidu powder developed by Inner Mongolia Runlong Biotechnology Co.,Ltd.was studied.According to the"PRC veterinary Pharmacopoeia"（part two）Chinese herbal medicines standards,the aim of this study is to provide a basis for the quality control of Qiqing baidu powder into production.Methods:The UV-visible spectrophotometric method was used to determine the main component chlorogenic acid of the main drug Lonicera japonica in Qiqing baidu powder.The test used thin-layer chromatography to identify the main drug Isatis tinctoria in the prescription.Results:The results showed that the thin-layer identification method had strong specificity,and the chlorogenic acid had a good linear relationship in the range of 13.2～66.0μg·m L^-1.The standard curve regression equation was:A=0.0373×C-0.0099（r=0.9999,n=6）.The chlorogenic acid content of the Qiqing baidu powder was1.763±0.0177μg·mg^-1.The average recovery of chlorogenic acid was 98.77±1.8757%,the recovery ranged from 96.6822%to 100.4405%,and the intra-and inter-day average precision were 0.356%and 0.096%,respectively,both less than 5%.Conclusion:The method used in the test is accurate and reliable,and does not require concentration correction.It can be used to study the quality standard of Qiqing baidu powder.