Investigation On Three Kinds Of Pig's Zoonotic Infection In Hunan Province

Chlamydia,Toxoplasma gondii and Brucella are three kinds of zoonotic pathogens that seriously threaten the life and health of human beings and many kinds of animals.Pigs play an important role in the transmission of these three kinds of pathogens,it is the common susceptible host of the three kinds of pathogens,and it has important public health significance for the study of zoonosis caused by these three kinds of pathogens.The first part of this study,indirect hemagglutination assay(IHA)was used to investigate the infection of Chlamydia in pigs in 14 cities(states)of Hunan Province.The results showed that the total positive rate of Chlamydia antibody in 3848 pig serum samples was 26.98%.By regions,the positive rates of Chlamydia antibodies in southern Hunan,northern Hunan,central Hunan and western Hunan were 25.63%,22.65%,32.78% and18.81%,respectively,Huaihua was the highest in local areas,reaching to 61.67%.The positive rates of antibodieswere 18.97%,25.40%,42.58% and 48.81% in spring,summer,autumn and winter in a year,respectively,it hasn't seasonality.The positive rates of piglets,fattening pigs,boars and sows were 10.90%,28.64%,48.39% and 33.63%,respectively,among them,the positive rate of piglets was the lowest and boars was the highest.The results showed that Chlamydia infection was prevalent in pig farms of Hunan province,which should be paid enough attention.The second part of this study,indirect hemagglutination assay(IHA)was used to investigate the infection of T.gondii in pigs of 8 cities(states)in Hunan Province.The results showed that 13 of 1259 pig serum samples were T.gondii in pigs positive,and the positive rate of antibody was only 1.03%.The infection of T.gondii was found in piglets,fattening pigs,boars and sows.The positive rates of T.gondii were 0.20%,0.28%,4.26%and 2.43%,respectively,with the highest among boars.The results showed that the infection degree of T.gondii in Hunan Province was low.The third part of this study used rose bengal plate agglutination test(RBT),serum agglutination test(SAT)and enzyme-linked immunosorbent assay(ELISA)to investigate the infection of Brucella in pigs in different scale pig farms in 14 cities(states)of Hunan Province.The results showed that 59 antibodies were positive in 2344 pig serum samples,and only one fattening pig serum was detected as positive,the positive rate was 0.04%;Two positive samples were detected by enzyme-linked immunosorbent assay(ELISA),the positive rate was 0.09%,one of them was still fattening pig and the other was sows.The investigation showed that the infection rate of Brucellawas very low in pigs in Hunan Province,the basic form is sporadic and does not cause large-area transmission,which is related to the severe prevention and control measures taken by Hunan Province,namely,one case was found to be destroyed immediately.The above results provide a reference for the prevention and control of three kinds of zoonosis in pigs farms in Hunan Province.