Serological Preliminary Investigation Of The Water Buffalo Neospora Caninum, Toxoplasma And Chlamydia In Some Areas Of Guangxi

Neospora caninum is a parasitic protozoan in a variety of animal cells. Bovine Neosporosis is a parasitic disease by Neospora caninum infection and parasitic on cattle. It is the most serious harm to cattle,causing huge economic losses to the cattle industry. So far, there is no effective drugs and vaccine to treatment Neosporosis, and the effective way of preventing and control Neosporosis is testing the infected cattle, then eliminated,to establish a no Neospora caninum infected cattle. Early diagnosis is an effective method of control the spread of the disease, so the research in diagnosis and epidemiological investigation of N. caninum is very important.Toxoplasma gondii is an obligate intracellular parasitic protozoan.The parasite host spectrum is wide, can infect about 300 kinds of mammals and more than 30 kinds of birds.Nearly one third of the global human population has been infected with T.gondii.Toxoplasmosis is an important zoonotic parasitic disease. T.gondii infection in human beings and animals is asymptomatic,there is no obvious clinical symptoms.In animal husbandry, Toxoplasma gondii can cause a variety of animal toxoplasmosis, and bring huge economic losses to the breeding industry.Chlamydia is an obligate intracellular parasitic microorganisms.The pathogen host is widespread. Chlamydia can infect almost all birds, animals and humans and cause a series of diseases to the host.Chlamydiosis is an important zoonotic parasitic disease.Chlamydia not only has a serious impact on human health, but also causes significant losses to the breeding industry.In the first part,the method of a competitive enzyme-linked immunosorbent assay(cELISA) was used to identify the prevalence of N. caninum infection in water buffaloes in Guangxi.The results showed that the positive rate of the N. caninum was 29.37%(222/756). Antibodies against N. caninum in different ages and feedings groups was not statistically different;Antibodies against N. caninum in different regions, seasons and years groups was statistically different(P<0.01).The seroprevalence of N. Caninum in water buffaloes in cow(52.94%)was significant higher then that in bull (25%, P<0.05). In the second part,the method of a Indirect blood coagulation(IHA) was used to identify the prevalence of T.gondii infection in water buffaloes in Guangxi.The results showed that the positive rate of the T.gondii was 2.05%(19/929).The positive rate of Toxoplasma gondii antibody in different seasons, sex, age and rearing patterns was not more than 10%.The infection rate of T.gondii in water buffaloes in Gulin,Baise,Qinzhou,Liuzhou,Yulin and Nanning was 3.03%(3/99),4.92%(3/61),1.95%(3/154),0(0/18),0(0/105) and 2.03%(10/492). The infection rate of T.gondii in water buffaloes in 2010,2012 to 2016 was 0(0/34),1.20%(3/249),5.26%(3/57),2.44%(1/41),2.67%(10/375)and 1.16%(2/173).In the third part,the method of a Indirect blood coagulation(IHA) was used to identify the prevalence of Chlamydiosis infection in water buffaloes in Guangxi.The results showed that the positive rate of the Chlamydiosis was 8.37%(75/896).Antibodies against Chlamydiosis in different ages, gender and feedings groups was not statistically different(P>0.05)Antibodies against Chlamydiosis in different regions and seasonsgroups was statistically different (P<0.01).Antibodies against Chlamydiosis in different ages was statistically different(P<0.05).This study is the first to investigate the epidemiology of the infection of the N. caninum,T.gondii and Chlamydia in water buffaloes in Guangxi.The results show that there is a potential risk for the transmission of these pathogens through water buffaloes in Guangxi. The results may provide the ’basic-data’ for further studies.