| The application of rare earths in agriculture is an important issue,and the mechanism of the analysis of the lack of chlorination of rice chloride has been reported.In this study,Zhongjiazao 17 was used as a test material to study the chlorophyll content,chlorophyll fluorescence parameters and resistance of rice seedlings with different concentrations of lanthanum chloride.The effects of oxidase activity system and root-related indicators,and transcriptome sequencing and analysis,in order to explain the molecular mechanism of lanthanum chloride regulation of rice seedlings.The main results are as follows:1.There are some differences in the effects of different concentrations of lanthanum chloride on the growth and development of rice seedlings.Long-term treatment with lanthanum chloride had no significant effect on the chlorophyll content and photosynthetic system II activity of rice seedlings,but had significant effects on leaf antioxidant enzyme activity system and root growth.When the concentration of lanthanum chloride is higher than 40 mg·L-1,the concentration of lanthanum chloride is further increased,and the activities of SOD,POD and soluble protein are significantly inhibited,and the inhibitory effect is stronger;root growth is hindered.The ultrastructure of cells was destroyed,which was not conducive to the growth and development of roots;there was no significant change in the effects of CAT enzyme activity and MDA content.When using lanthanum chloride in the growth period of rice seedlings,the concentration should not exceed 40 mg·L-1.2.With normal samples as control,417 and 1907 genes were significantly differently expressed in rice treated with 2 mg·L-1and 200 mg·L-1lanthanum chloride,respectively.The expression of 1968 differential genes showed a significant trend.Two of the four different significant trends were significantly up-regulated,two were significantly down-regulated,and all significant trends were divided into 2 mg·L.Differential expression at 200 mg·L-1and no differential expression at 2 mg·L-1,and differential expression at 200 mg·L-1.3.The GO functional enrichment analysis of the differential genes expressed in the four significant trends showed that after treatment of rice seedlings by lanthanum chloride,the pathways involved in membrane transport,membrane metabolism,photosynthesis and metabolism in rice were significantly down-regulated,and related signals were transferred.The genes involved in hormone synthesis,regulation and regulation,and antioxidant enzyme activity are significantly up-regulated.The combined effect of these genes is an important reason for the change of rice enzyme activity system.The results of significant enrichment analysis of KEGG metabolic pathways by differentially expressed genes in four significant trends indicated that after treatment of rice seedlings with lanthanum chloride,?-glucosidase,cinnamyl alcohol dehydrogenase,and pine aldehyde dehydrogenase were The up-regulated expression of genes involved in lignin synthesis increases the amount of lignin synthesis and plays a positive role in the elimination of reactive oxygen species in rice.4.Spraying lanthanum chloride solution on rice under two different irrigation modes in different periods can significantly improve the activity and yield of rice antioxidant enzyme system,and the effect of lanthanum chloride on antioxidant enzyme system and rice fluorescence parameters at booting stage Large,more sensitive to moisture.Spraying 20 mg·L-1concentration of lanthanum chloride can increase the activity of antioxidant enzyme system and the content of osmotic adjustment substance under water shortage irrigation and conventional irrigation at booting stage.Spraying 100 mg·L-1concentration of lanthanum chloride can increase the absorption and utilization of light energy by PSII light system at the booting stage and the conventional irrigation,thereby enhancing the photosynthesis ability of the plant,promoting growth and development,and ultimately increasing yield. |
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