Cultivation Of Se-enriched Jujube And Application Of Se-enriched Technology

Selenium(Se),as an essential trace element of the human body,has multiple biological effects.In order to solve the problem of selenium deficiency worldwide,selenium supplementation is necessary.This experiment takes jujube as the research object,cultivates selenium-rich jujube by injecting selenium-rich nutrient solution,and studies the characteristics and morphology of selenium-rich jujube by adopting selenium-rich technology.Concluded as follow:1.This experiment used the HG-AFS method to determine the total selenium content of selenium-enriched jujube.The results showed that the total selenium content of jujube leaves was always higher than that of jujube,and the total selenium content of jujube leaves reached 0.8914 mg/kg.The dry-fresh ratio of selenium-rich jujube was 45.73%,and the selenium content of jujube powder was always higher than that of fresh jujube.The highest total selenium content in jujube powder and fresh jujube was 0.7029 mg/kg and 0.6211 mg/kg,respectively,which were80.83% and 79.28% higher than that in the blank group.Therefore,selenium-enriched jujube cultivated by injecting an appropriate amount of selenium nutrient solution can be used as an effective selenium supplement product.2.The experiment selected ultra-pure water,HCl,NaOH,and Tris-HCl as extractants to study the contents of selenium in different dissolved states in Se-rich jujube.The results showed that there were four kinds of dissolved selenium distribution rules: alkali-soluble state> water-soluble state> Tris-HCl dissolved state>acid-soluble state.This shows that the selenium-enriched jujube has the strongest selenium migration and transformation ability under the alkaline condition.3.The optimum extraction conditions for soluble protein and soluble polysaccharide in selenium-enriched jujube were obtained by single factor experiments.Under the optimal extraction conditions,the maximum contents of soluble proteins and polysaccharides in selenium-rich jujube were 6.157 g/kg and71.58 g/kg,respectively,and the maximum contents of protein selenium and polysaccharides were 0.0357 mg/kg and 0.1622 mg/kg,respectively.The results showed that the ability of polysaccharide to bind selenium was stronger than that of protein.4.The biological activity of selenium-rich jujube was measured by UV method.The results showed the maximum reducing power of selenium polysaccharide and selenoprotein was 88.13% and 95.62% respectively,and the highest DPPH radical scavenging rate was 89.25% and 98.75% respectively.The results showed that the biological activity of polysaccharide selenium was higher than that of protein selenium.5.Quantitative analysis of various trace elements in selenium-enriched jujube by ICP-MS was carried out to study the biological stress of each element.Studies have shown that,with the gradual increase in the amount of selenium,selenium has a significant synergistic effect on the content of Zn,Mn,Cu,Ca,Fe,V,Mo,and Co;and selenium has significant effects on heavy metal elements such as As,Cd,Hg,and Pb.Antagonism.6.Qualitative and quantitative analysis of organic selenium and inorganic selenium in selenium-rich jujube by HPLC-ESI-MS.The results showed that Se-Met was mainly present in selenium-enriched jujube with the highest content of 16.91 μg /kg,and inorganic selenium was not present.It shows that jujube can transform inorganic selenium into organic selenium for human body absorption and utilization.