Screening Of Varieties With High Contents Of Active Ingredients In Dendrobium And Their Cultivation And Reproduction Techniques

Dendrobium is a perennial herb of the Orchidaceae family.There are 74 species and 2 varieties of Dendrobium in China.Some Dendrobium plants are traditional medicinal and ornamental plants in China and have a long history of application.Dendrobium species are extremely abundant,but germplasm resources are relatively scarce.Dendrobium has extremely high medicinal value.In the 2015 edition of "Chinese Pharmacopoeia",the content of erianin with anticancer effect was used as the quality evaluation index of Dendrobium chrysotoxum.Due to the huge demand in the market,the resources of wild Dendrobium are endangered.As a result,the variety of Dendrobium on the market is complicated,and there are a large number of false Dendrobium or inferior Dendrobium.Therefore,the quality identification and artificial cultivation of Dendrobium plants are extremely important.In view of this,this study collected 38 sppecies of Dendrobium resources,firstly based on the measurement of their morphological indicators,a simple classification,in order to quickly and intuitively identify;secondly,the classification and integration of multiple names of some Dendrobium;The thesis focuses on the determination of the38 species of Dendrobium based anti-cancer core component erianin content,including the corresponding water content determination.The tissue culture technique of Dendrobium nobile and Dendrobium officinale with high medicinal value was studied by tissue culture technology.(1)The morphological indexes of 38 species of Dendrobium were measured by observation and measurement.In this experiment,three categories of plant morphology,stem morphology and leaf morphology were briefly classified.According to the morphology of the plant,it can be divided into three types: upright,semi-upright,and drooping;according to the morphology of the stem,it can be divided into three forms: cylindrical,spindle-shaped,and rod-shaped(the stem is enlarged,and the upper or lower parts are thinner).According to the leaf morphology,it can be divided into lanceolate,oval and sword shapes.(2)By referring to the literature and investigating,the synonyms and hybrids of these 38 species of Dendrobium are classified and integrated.Among them,ninespecies of Dendrobium have synonyms and two are hybrids(3)The water content of 38 species of Dendrobium was determined by the drying method,and the water content was higher than 50%.Among them,Dendrobium huoshanense had the highest water content,reaching 89.76%.The lowest water content is 52.07% of Dendrobium ellipsophyllum.(4)The content of anti-cancer component erianin in these 38 species of Dendrobium was determined by high performance liquid chromatography.The results showed that the content of 0.047% erianin(calculated as dry product)was detected in Dendrobium chrysotoxum.At the same time,the experimental results show that the determination method of erianin established in this study is simple,fast and the results are intuitive.(5)Using Dendrobium officinale and Dendrobium nobile stem sections as explants,a tissue culture rapid propagation system was established through tissue culture techniques.The results showed that the optimal medium for Dendrobium officinale bud induction was MS + 3mg/L 6-BA + 0.5mg/L NAA + 30g/L sucrose +7g/L agar + 1g/L activated carbon,the optimal medium for bud proliferation is MS +1.0mg/L 6-BA + 0.5mg/L NAA + 30g/L sucrose + 100 g /L banana puree,the optimal medium for rooting is MS + 1.0mg/L IBA + 1.0mg/L NAA +30g/L sucrose + 7g/L agar + 1g/L activated carbon.The optimal medium for Dendrobium nobile bud induction is 1/2MS +2mg/L 6-BA + 0.5mg/L NAA + 30g/L sucrose+ 7g/L agar +1g/L activated carbon,the optimal medium for bud proliferation is 1/2MS + 3.0mg/L6-BA + 0.5mg/L NAA + 30g/L sucrose + 100 g /L banana puree,the optimal medium for rooting is 1/2MS + 0.6mg/L IBA + 0.3mg / L NAA.30g/L sucrose+ 7g/L agar +1g/L activated carbon.