Differential Expression Of Root Genes In Cerasus Pseudocerasus Lindl. 'Manaohong' Treated With Biochar

Biochar is an insoluble solid substance produced by high temperature thermal cracking of agriculture and forestry wastes under the condition of hypoxia or no oxygen.On the one hand,biochar contains an amount of mineral elements(such as N P K and some trace elements),which can provide a better supply of elements for plant growth and development.On the other hand,its special pore structure can provide a good environment for soil microorganisms,regulate their growth,development and metabolism and improve soil fertility.Cerasus pseudocerasus Lindl.‘Manaohong'is an excellent cultivar with long shelf life and the planting area has exceeded 16,666 hm2 in Guizhou,which is of great economic value.In the present study,the research found that the addition of straw biochar in soil significantly promoted the physiological growth of ‘Manaohong'.Therefore,this study mainly explored the molecular mechanism of the effect of biochar on roots by transcriptome analysis of ‘Manaohong' seedlings under biochar treatment.At the same time,the introduction of "non nutritive" biochar was carried out to explore the contribution differences of biochar structure characteristics and nutrient composition characteristics in the process of its function.The main research contents were as follows:1.Through transcriptome sequencing,a total of 154,724 Unigene were obtained from transcriptome data in June and September.It was annotated in 6 databases including NR,Swiss-PROFT,Pfam,GO,COG and KEGG to obtain the main enriched metabolic pathways of these Unigene.2.In the transcriptome data of June and September,12 unigenes were randomly selected for qRT-PCR validation,and the change trend of expression was consistent with the transcriptome.By comparing the transcriptome data of different treatments,and enriching KEGG and go genes,the metabolic pathways of flavonoid biosynthesis,plant hormone signal transduction pathway and phenylpropionic acid biosynthesis were obtained.3.The result shows that there were differentially expressed genes between the two experimental treatment and one control treatment:Compared with the control treatment,a total of 5,715 DEGs were screened in June and 544 in September in the "nutrient" biochar treatment;3,363 DEGs were screened in June and 279 DEGs were screened in September in the "nutrient" biochar treatment.Compared with the non nutritive biochar treatment,1,536 DEGs were screened in June and 309 DEGs were screened in September.4.Chalcone synthetase gene was cloned from cherry by Nested-PCR,namd CpCHS2(Gene bank:MT112906).The total length of the CpCHS2 cDNA sequence was 1,176 bp,containing 391 amino acid.CpCHS2 protein are hydrophobic protein and have no transmembrane region.It is mainly located in cytoplasm.Evolutionary analysis showed that CpCHS2 cherry has the highest homology similar with prunus cerasifera and prunus persica.5.qRT-PCR analysis showed that different genes were expressed in different tissues or months.contrast to the nutrient biochar treatment,the expression levels in root and leaf were higher than those in "non nutrient" biochar and control group in different developmental stages.