Functional Analysis Of Wheat(Triticum Aestivum L.) Vernalization Related Gene Wcor18 And Photoperiod Related Gene TaUb27

In recent years,with the development of molecular biology of photoperiod and vernalization in wheat,many photoperiod and vernalization genes have been discovered,but its can not explain the variety type of wheat photoperiod and vernalization development.In this paper,based on the gene expression profile of vernalization of wheat,a vernalization expression differential gene Wcor18 was screened,and its overexpression vector was constructed.At the same time,we identified and analyzed the overexpression plants of Ta Ub27,which is a photoperiod related gene,and carried out a variety of stress resistance experiments.The results are as follows:1.The ORF of Wcor18 gene is 498 bp,which encodes 165 amino acids,including 23 acid amino acids(ASP + Glu)and 14 basic amino acids(Arg+ Lys).Its theoretical Pi is 4.93.The predicted Wcor18 protein is a hydrophilic protein with a destabilization index of 44.64,which belongs to an unstable protein and is easy to hydrolyze and oxidize.2.The results of semi quantitative PCR and fluorescence quantitative PCR showed that the expression of Wcor18 gene was up-regulated in low temperature environment.On the basis of verifying the up regulation of Wcor18 gene induced by low temperature,the Wcor18 gene was cloned and transformed,and the transgenic progenies of Jing 841 wheat were obtained.It was found that the young spike development of transgenic progenies was accelerated,and vernalization was completed through the double ridge stage ahead of time,which indicated that Wcor18 gene could accelerate the vernalization development of wheat.Under 4 ? low temperature,20% PEG6000,ABA and Na Cl,the expression of Wcor18 gene was increased by fluorescence quantitative PCR,which indicated that Wcor18 gene was involved in the process of stress.3.The expression of Ta Ub27 was higher in light than in dark.The response of varieties to day length was different.The expression of Ta Ub27 in Liaochun 10 was less affected by day length,that is,under long day and short day conditions,the expression of Ta Ub27 induced by light condition was high;while Ningchun 36 was greatly affected by day length,and the expression of Ta Ub27 was induced by long day,and the short-day light-induced expression effects are not obvious.4.By observing the Ta Ub27 gene overexpression transgenic wheat lines,we found that the Ta Ub27 gene transgenic lines were earlier than the control in heading stage,booting stage and mature stage,which indicated that Ta Ub27 gene promoted the development process of wheat in the photoperiod development stage.It was speculated that Ta Ub27 gene promoted the photoperiod development of wheat.Analysis of tissue-specific expression of Ta Ub27 showed that the expression level of Ta Ub27 was the highest in leaves and almost no expression in roots and ears,which was consistent with the photoperiod response organs of wheat.5.The expression of Ta Ub27 gene was detected by fluorescence quantitative PCR under Na Cl,20% PEG6000,ABA and 4 ?,respectively.It was found that the expression level of Ta Ub27 gene increased with the treatment time and reached the maximum at 24 h.Further analysis of the effect of transgenic overexpression plants on the germination rate under high salt conditions showed that the germination rate of the transgenic plants under high salt conditions was significantly higher than that of the control,suggesting that Ta Ub27 gene may play a role in wheat resistance to stress.