Isolation And Functional Characteristics Of Intestinal Microbes In Mongolian Horse

In this study,the contents of intestinal six parts of a male Mongolian horse aged one-and-a-half-year-old collected from the horse slaughterhouse in Hohhot,Inner Mongolia were taken as the research object.Its strains were isolated,and the strains were tested for exopoly saccharides production,cholesterol degradation,DPP-IV inhibitor production,acid and bile salt resistance,oxidation resistance,reduction and hydrophobicity tests.Strains with good functional characteristics were screened out,which provided the target strains and scientific basis for further research on functional characteristics of intestinal microbes in Mongolian horse.In the study,91 strains were isolated and purified,including 20 strains of lactic acid bacteria,31 strains of yeast,and 40 strains of bacillus.The screened strains were tested for exopoly saccharides production,cholesterol degradation,and DPP-IV inhibitor production by using methods such as phenol sulfuric acid.The test screened out a total of 10 strains of MW la,MM1,MB3,YF1a,YW4a,YF3a,YB4,LF2,LH2a,and LW1,they had the ability with different degrees of exopoly saccharides production,cholesterol degradation,and DPP-IV inhibitor production.The strains with the above functional characteristics were subjected to acid resistance bile salt resistance test,oxidation resistance test,reduction test and hydrophobicity test.Strains MW1a,MM1,MB3,YW4a,YB4,LH2a,LW1 had the above functional characteristics.Among them,the comprehensive ability of strain YW4a was the best,and deeper research in the later period can be carried out.