Screening And Identification Of Target Genes Of AtbZIP2 In Arabidopsis Thaliana

Being sessile,plants have innate immune system to protect themselves against pathogens infection.Transcription factors(TFs)play key roles in mediating their downstream target signaling pathways in plant defense,especially the b ZIP transcription factors family given that found to be involved in the whole life of plant growth.Previous research by our project group found that AtbZIP2 transcription factor is involved in the defense response of Arabidopsis.Based on this study,we further analyze the mechanism of AtbZIP2 regulating plant disease resistance.In this study,three genes associated with JA signaling pathways were screened based on Arabidopsis mutant RNA-seq: JAZ5,JAZ6,JAZ7.The analysis result of the promoter region of these three genes by online software plant CARE,found that they all contain G-box cis-acting elements that may be recognized by b ZIP transcription factors.AtbZIP2 transcriptional activation was found through yeast expression system.Yeast one hybrid results show that AtbZIP2 may interact with JAZ6 promoter DNA but not with JAZ5,JAZ7 promoter DNA.The deletion mutation of the JAZ6 promoter sequence: JAZ61(-402bp),JAZ62(-300bp),JAZ63(-201bp),JAZ64(-102bp),found that AtbZIP2 does not interact with JAZ61,JAZ62,JAZ63,JAZ64.Together,we found that the AtbZIP2 transcription factor interacts with the JAZ6 promoter DNA,and the binding site of AtbZIP2 and the JAZ6 promoter fragment may in the region from-579 bp to-400 bp upstream of the ATG in the JAZ6 promoter region.And the mechanisms that govern how Arabidopsis AtbZIP2 involved in plant disease resistance enriched at the transcription level.