Correlation Between Syndrome Differentiation And Clinical Indicator Of IBD

Infectious bursal disease(IBD)is a highly infectious and immunosuppressive disease caused by the infectious bursal disease virus(IBDV)that affects the global poultry industry.It has been known as the three major infectious diseases of poultry industry with Newcastle disease and Avian Influenza[1]At present,traditional Chinese medicine has certain advantages in the treatment of IBD,and have also been obtained some achievements,but due to the diverse clinical manifestations and the prognosis varies greatly of IBD.Therefore,this study established IBD disease model by infected SPF chicken with IBDV JS strain,combined with the theory of traditional Chinese veterinary medicine(TCVM)and the judgment criteria of traditional Chinese veterinary syndrome type,to carry out initially differentiate of symptoms of IBD,and then observed the pathological examination and pathology of chickens infected with IBDV histological and clinical indicators,introduce them into the TCVM veterinary syndrome,exploring the relationship between the TCVM veterinary syndrome and clinical indicators,providing an objective basis for the prevention and treatment of IBD and TCVM veterinary syndromes,as well as the classification and prognosis of IBD and further treatment lay the foundation of science.1.Syndrome differentiation of infectious bursal disease of chickenSPF chickens were randomly divided into two groups(blank group and IBD model group),and IBDV JS strain was used to infected SPF chickens in order to establish IBD disease model.By observed the clinical symptoms of SPF chickens and measured their body weight and body temperature everyday;and combined with the judgment standards of the Wei Qi Ying Xue model and spleen-qi deficiency model developed in this study,based on syndrome differentiation obtained the initial diagnosis of IBD.The results showed that the chicks began to show clinical symptoms 24 h after IBDV infection,the most typical between 48 h and 72 h,the symptoms of sick chickens gradually reduced after 108 h,and only 1/4 of the chickens had clinical symptoms at 120 h,The symptoms of sick chickens basically disappeared at 144 h.The chicks began to die 2 days after infection,and the deaths were concentrated between the 2nd and 5th days after infection.The body temperature decreased briefly at 12 h,108 h,and 132 h,and the overall body temperature of the flock was higher than that of the normal group between 24 h and 96 h.In summary,infectious bursal disease is a warm disease of exogenous epidemic disease,and its development law is accord with the transmission and differentiation of Wei Qi Ying Xue syndrome.Based on the differentiation of syndromes of Wei Qi Ying Xue,IBD belongs to the Weifen syndrome between 24 h and 48 h,and belongs to the Yingxue syndrome between 48 h and 120 h.According to organ differentiation,IBD belongs to the syndrome of deficiency of spleen qi,and the spleen deficiency of spleen qi deficiency is not smooth between 24 h and 48 h,and the spleen of spleen qi deficiency is not unified between 48 h and 120 h.The evolution rule of IBD is that the disease occurs in Weifen first,and then occurs in Yingfen.The lesion center is mainly spleen and stomach qi deficiency.2.IBD's pathological and pathological dynamic observationSPF chickens were randomly divided into two groups(blank group and IBD model group),and the IBDV JS strains was used to infected SPF chickens in order to established IBD disease model,which was measured by immune organ index measurement,necropsy lesion observation,tissue section HE staining observation,The pathology of various organs of SPF chicken infected with IBDV was dynamically observed.The results showed that 24 hours after IBDV inoculation,hemorrhage of thymus and leg muscles,hemorrhage of splenomegaly and bursa of bursa;The liver and kidney was khaki and bleeding after 48 h;The pericardium becomes thinner,the heart is white,swollen duodenal hemorrhage,cecal enlargement and other lesions after 60 h,and the stomach only bleeding after 72 h;among the lesion and histological changes are lighter between 24 h and 48 h,the condition was severed and the lesions worsened after 60 h,which was consistented with the syndrome differentiation results in Chapter 1.3.Correlation between IBD and clinical indicators of spleen deficiencySPF chickens were randomly divided into two groups(blank group and IBD model group),and then IBDV JS strain was used to infect SPF chickens to establish an IBD disease model.The blood of SPF chickens was collected aseptically,and the hematology and oxidative damage indexes of spleen-qi deficiency were measured The results showed that the serum TBLL of the chicks of the model group was higher than that of the blank group at 48 h(P<0.05),and was lower than that of the blank group between 60 h and 168 h;both serum TP were ignificantly lower the blank group(P<0.05)and serum ALB were extremely significantly lower the blank group(P<0.01)during the experiment;serum GLO was no change significantly within 120 h after infection,and was significantly lower than the blank group between 120 h and 168 h(P<0.01);Although the serum CK was higher than the blank group,it was not statistically significant.the total number of leukocytes in the blood of the model group chickens decreased from 24 h to 84 h(P<0.01),and significantly decreased at 96 h(P<0.05);the red blood cells in the blood were significantly between 60 h and 144 h lower than the blank group(P<0.05).The serum SOD was decreased significantly from 72 h to 96 h(P<0.01);the serum GSH-PX was decreased from 72 h to 120 h(not statistically significant at 84 h),and the limit value was decreased(P<0.01);It increased significantly from 96 h to 144 h(P<0.01).The results show that the reduction of TP,ALB,SOD,GSH-PX,WBC and RBC after IBD infection be used as clinical indicators of spleen-qi deficiency disorders.