Study On The Propagation Technology Of Spores Of Osmunda Cinnamomea L. Var. Asiatica

The research studies the spore germination,prothalli increment and sporophyte transformation of Osmanthus cinnamomea（also called Osmunda）,a special forest sig-htseeing wild vegeTab.in Changbai Mountain.It is intended to provide more seedlings and industrial technical support for artificial bionic cultivation in Changbai Mountain area,at the same time enriching the diversity of ornamental fern species in the city.The research results are as follows:1.The most suiTab.method for spore sterilization of Osmunda is to use 0.1%mercuric chloride solution.After being sterilized for 8 min,the spore germination rate is up to 61.11%;During explants collection,the darkgreen,arc-shaped spores with obvious granular spores wre selected.The germination started around 17d with a genrmination rate of 77.78%.The spores were cut into small pieces of about 1 cm×1 cm and inoculated directly into the medium.After19 days,the germination rate was 80%;the state of crushing and inoculation was not good.he germination time of spores was the shortest and the germination rate was the highest when soaked for 24 h at 100 mg·L^-1 IAA,ie 7 d,90.27%;GA₃ is not suiTab.for pretreatment of hormones.2.The optimum inorganic salt and sucrose concentration of induced protoplasts were1/4MS and 0 g·L^-1,respectively,and the induction rate was 87.79%.The most suiTab.hormonal conditions were 1/4MS+KT5.0 mg·L^-1+NAA0.3 mg·L^-1.The induction rate was 88.55%.3.The optimum conditions for the proliferation of Osmunda leaf body were:1/2MS+15g·L^-1sucrose+0 g·L^-1 agar,with a value-added coefficient of 8.83 and in good condition.The concentration of photosynthetic enzyme was positively correlated with the proliferation coefficient of protoplast（P<0.05）,and the highest proliferation coefficient was 9.13 when the concentration was 300 times.The best hormone combination for protoplast proliferation was 1/2MS+KT5 mg·L^-1+GA₃3 mg·L^-1+NAA1.5 mg·L^-1;The use of monochromatic illumination in the value added process tends to cause browning of the original leaf body,and white light was more conducive to its growth and development.4.When 1/2MS+15 g·L^-1 sucrose was used in sporophyte transformation,the sporophyte grew well,and the transformation rate reached 42.04%.The optimal combination of hormones for sporophytic transformation was 1/2MS+KT7 mg·L^-1+GA₃2 mg·L^-1+NAA1 mg·L^-1+6-BA0.5 mg·L^-1.The sporophytic conversion rate of"photosynthetic enzyme"treatment was higher than that of CK.When the concentration was 500 times,the spore body growth was the best,and the conversion rate was 57.23%;Agar additionhad no significant effect on the transformation rate of sporophyte,but sporophyte growth grew better without agar.Five kinds of substrates were used for the transformation outside the bottle.Among them,coconut slag+perlite+grass charcoal（1:1:2）mixed matrix,the highest conversion rate of sporophytes was 68.72%,followed by Coconut slag 42.15%;aga-in perlite+grass charcoal（1:3）was 40.61%.5.Rooting was induced by 1/2MS+IBA0.5 mg·L^-1 medium,and the rooting rate was93.33%after 40 days.The optimal substrate for sporophyte transplanting was coconut slag+perlite+peat（1:1:2）mixed matrix,and the highest survival rate of transplanting was 85%,fol-lowed by the survival rate of coconut slag（80.83%）;The substrate containing the mountain soil had the lowest survival rate,only 37.5%,and it was prone to brown spots and even blackened to death.