Proteomics And Lysine Acetylation Proteomics Analysis Of Chicken Cecum During The Schizogony Of Eimeria Tenella

Coccidiosis in chicken is an intestinal disease caused by Eimeria.Coccidiosis in chicken causes huge losses to the world's poultry breeding industry every year.Currently,the prevention and treatment of coccidiosis in chicken Mainly drug control and vaccine control,but the emergence of drug resistance caused by the abuse of anticoccidial drugs makes the development of new drugs imminent.The life history of coccidiosis in chicken is complex,involving different morphological and host cells,and its development mainly through three stages: sporogony,schizogony and gametogamy.Schizogony is the asexual reproduction stage,Which is accompanied by the invasion of host cells,proliferate in host cells,and destroy host cell.During the invasion process,the coccidia and hosts will change there proteome,and the protein is the performer of physiological functions.The study of protein structure and function directly clarifies the physiological and pathological changes of the species.Therefore,study the changes of host proteins during Schizogony is great significance for elucidating the pathogenesis of coccidia,discovering potential drug targets and immune control.This study collected normal cecal and merogony cecal(E.tenella infection 120 hours)in chicks.The cecal samples were by protein extracted,trypsin,TMT,HPLC,enrichment,LC-MS/MS analysis,database search,followed by bioinformatics analysis using protein annotation,protein functional enrichment,cluster analysis based on protein functional enrichment,and protein interaction network analysis.In this experiment,we used the TMT quantitative protein technology to study the proteomics and acetylation modification of the samples.A differential expression analysis of quantitative proteins revealed 717 differentially expressed proteins,of which 373 were up-regulated and 344 were down-regulated.Subsequently,we performed protein annotation,functional classification,functional enrichment analysis,cluster analysis and protein interaction network analysis for the identified of differentially expressed proteins.Analysis results show that differential proteins are roughly related to cytoskeleton,apoptosis,inflammatory response,metabolism and function,and ribosome.The amount of protein expression in the cytoskeleton of the host changes(spectrin,ankyrin 1,centrosome-related proteins,etc.),revealing that the invasion of Eimeria tenella may affect the host cytoskeleton;also the up-regulation of the key effector substances Caspase8 and Bcl-2 in the apoptotic pathway,revealing that Eimeria tenella may mediate the host apoptosis of cells facilitates the increase of merozoites.In conclusion,Eimeria tenella may facilitate its proliferation by regulating host cell apoptosis,inflammatory response,metabolism,etc,while down-regulating the corresponding immune response to evade host clearance.This provides information on understanding the molecular mechanisms of Eimeria tenella and finding new drug targets.The acetylation modification plays an important role in metabolic regulation,apoptosis and signal transduction.We also used the TMT technique to analyze the differentially acetylated modified protein in the comparison group,and obtained 150 differentially acetylated modified proteins.Among them,80 were up-regulated and 70 were down-regulated,and 211 differential acetylation sites were identified on these acetylated proteins,of which 108 were up-regulated and 103 were down-regulated.Through functional classification,functional enrichment analysis,protein interaction network analysis and cluster analysis of differential acetylated proteins.The analysis showed that acetylation proteins like 4E-BP1,Cullin3-E3 ubiquitin ligase,hexokinase 2,guanylate-binding protein 1 and thrombospondin 1 are mainly related to cell cycle,apoptosis and signal transductionthese.Prompt that Eimeria tenella may regulate the cell cycle,apoptosis and metabolism of host cells by acetylation modification to facilitate their development.Interestingly,we identified that acetylated proteins are down-regulated in a novel cell death mode Ferroptosis pathway,which may be a new target for screening drugs.These data fill the gap in protein acetylation after Eimeria tenella infection in the host,providing a basis for a better understanding of the interaction between Eimeria tenella and the host.