Expression Analysis Of D Subfamily Of BZIP In Cucumber And Preliminary Analysis The Function Role Of CsbZIP120

Plant bZIP has important regulatory effects on plant growth and stress response,but there are only a few reports on bZIP transcription factors in cucumber.In this paper,bioinformatics methods were used to identify bZIP family members and D subfamily members in cucumber,and the expression patterns of D subfamily members under abiotic and botrytis stress were analyzed.Meanwhile,D in response to Botrytis cinerea whose function role of CsbZIP120 was initially explored.The main results are as follows:Based on cucumber genomic information,62 CsbZIP family members were identified,which have typical bZIP motifs,which were classified into nine sub-families: A,C,D,E,F,G,H,I,S according to their sequence homology and conserved domain characteristics.,there are 9 D sub-family members.Expression levels of D subfamily CsbZIP71,CsbZIP420 and CsbZIP84 were significantly up-regulated after NaCl stress treatment;CsbZIP33,CsbZIP73 and CsbZIP420 had obvious responses to PEG osmotic stress treatment.CsbZIP26 and CsbZIP71 have a significant response to methyl viologen treatment.CsbZIP26 and CsbZIP33 were induced by methyl viologen and salicylic acid,and the expression increased significantly in the late stage of methyl treatment.CsbZIP26 and CsbZIP120 strongly responded to gray mold stress.CsbZIP120 genetic transformation was carried out on Arabidopsis thaliana,and T3 generation homozygous transgenic line OE(over expression)was obtained.The three-deficient mutant tga2/5/6 mutant and OE in Arabidopsis thaliana were identified by gray mold inoculation.It was found that the tga2/5/6 mutant lesion was larger than the wild type,and the OE lesion area was significantly smaller than the wild type.