Preliminary Study On The Protective Effect Of Recombinant Saccharomyces Cerevisiae Expressing VP28 White Spot Syndrome Virus

White spot syndrome virus(WSSV)is one of the most dangerous pathogens infecting shrimp and other crustaceans.It has the characteristics of wide host range,high fatality rate and strong infectivity,which causes great economic losses to the world shrimp breeding industry.Unfortunately,there is no effective drug to prevent WSSV infection,which can only be prevented by strengthening management measures.Undoubtedly,the development of effective drugs is one of the most effective measures to prevent WSSV infection.In this study,recombinant S.cerevisiae EBY100/PYD1-VP28 was constructed based on the envelope protein vp28 gene of WSSV(gene bank number:FJ756456.1)was used as the research object.The expression of S.cerevisiae EBY100/PYD1-VP28 was analyzed by SDS-PAGE,Western blot,immunofluorescence analysis and flow cytometric assay.Then,Penaeus vannamei was used as an animal model and S.cerevisiae EBY100/PYD1-VP28 was orally administrated,the protection efficacy of S.cerevisiae EBY100/PYD1-VP28 was determined by WSSV challenge experiment.S.cerevisiae EBY100/PYD1-VP28 was successfully constructed.WSSV challenge experiment indicated that the survival rate of Penaeus vannamei was 60% after oral administration.This implied that recombinant S.cerevisiae EBY100/PYD1-VP28 could be applied for shrimp aquaculture to prevent WSSV infection,which has a huge market application.