Genetic Diversity And Relationship Analysis Of Hazelnut Germplasm Resources Based On SSR,SRAP Marker

The hazelnut is rich in nutrition and unique in flavor,and has high nutritive value,economic value,medicinal value and commercial value.The study value of the hazelnut can be more highlighted by the extraction of taxcl from the leaves and the trunks.SSR and SRAP molecular markers were used to analyze the genetic diversity and relationship of hazelnut resources,It provides a theoretical basis for the protection of hazelnut resources,the utilization of high-quality germplasm resources and the breeding of new varieties in China.The main results are as follows:1.32 pairs of primers and 28 pairs of primer combinations with clear bands and rich polymorphism were selected for analysis of hazelnut genetic diversity and relationship;The annealing temperature of 28 pairs in SSR primers were screened,and the SSR-PCR and SRAP-PCR systems were optimized respectively.2.The average percentage of polymorphic loci of SSR and SRAP primers were 88.14% and 100%,respectively.The results showed that the two molecular markers could be effectively amplified.So They could be used to study the genetic diversity of hazelnut resources.The main value of SSR primers: allele number,effective allele number,Shannon index and Nei’s genetic diversity index were 4.8125,3.6576,1.3803 and 0.714,respectively.The average number of alleles,effective alleles,Shannon index and Nei’s genetic diversity index of SRAP were 1.8904,1.6468,0.5459 and 0.3701,respectively.The diversity index of both molecular markers showed that the genetic diversity of hazelnut had a relatively high level.3.Based on the genetic diversity index of SSR molecular markers among Corylus species,the genetic diversity of different species of Corylus was in the order of C.heterophylla> C.heterophylla× C.avellana > C.chinensis > C.kweichowensis > C.avellana > C.mandshurica> C.fargesii.Based on SRAP molecular marker,the genetic diversity index of Corylus species shows that the order of C.avellana > C.heterophylla > C.heterophylla× C.avellana > C.kweichowensis > C.chinensis > C.fargesii.Both of the two molecular markers showed that the genetic diversity of Chinese hazelnut was the highest in C.heterophylla,the lowest of C.fargesii.4.The results of molecular variance analysis with SSR markers and SRAP markers showed that most of the genetic variations of the Hazelnut occurred within the population(SSR=84%,SRAP=89%),and a few genetic variations occurred in the inter population.5.According to the genetic distance and genetic consistency of SSR and SRAP markers,the genetic relationship was the closest between C.heterophylla and C.heterophylla× C.avellana,and the genetic relationship was the farthest between C.fargesii and C.ferox var.thibetica.and that between C.heterophylla and C.mandshurica was closer.The genetic relationship of C.ferox var.thibetica was close to that C.kweichowensis and C.chinensis.The genetic relationship of C.yunnanensis and C.ferox var.thibetica is far from that of other Corylus species.There is a certain correlation between the clustering results and the source of materials.Although the two markers could not distinguish all the Corylus species completely,the clustering results could reflect the genetic diversity and genetic relationship of the hazelnut resources to a certain extent.PCo A was similar to the UPGMA clustering results.