Determination Of Nutritive Value Of Barley Green Fodder Cultivated In Hydroponic System

In order to learn the nutritional value of barley grass?Hordeum Vulgare L.?,Barley grain was sprouted in a still hydroponic growing chamber in the 4^th,5^th,6^th and 7^thh days periods and sampled for determination of basic nutrients,minerals and 18 kinds of amino acid content and analysis of its components using Cornell net carbohydrate-protein system?CNCPS?.The nylon-bag method was used to study the degradation of various nutrients in the rumen of dairy cows,and the in vitro gas production method was used to evaluate the barley grass in different hydroponic time.Nutritional value provides basic data for the rational use of green barley grass in herbivores.Experiment 1:This experiment evaluated the nutritional value by measuring the nutrient content of hydroponic barley grass.The results showed that:1)The contents of in the 7^th day CP,NDF,ADF,NDIP,ADIP,SOLP and NPN were dramatically significantly higher than in the 4^th,5^th,and 6^th days?P<0.01?.2)The contents of in the7^th day Ca,Mn and Fe were dramatically significantly higher than in the 4^th,5^th,and 6^thh days?P<0.01?.3)The levels of Lys,TAA and EAA in the 7^th day were dramatically significantly higher than in the 4^th,5^th,and 6^th days?P<0.01?.4)The content of medium PB₂ at the 7^th day was dramatically significantly higher than in the 5^th,and 6^thh days?P<0.01?.The content of PB₃ and PC were significantly higher in the 7^th day than in the 4^th day?P<0.05?.The content of CNSC in the 7^th day was dramatically significantly lower than in the 4^th,5^th,and 6^th days?P<0.01?.The content of CB₂ in the7^th day was dramatically significantly higher than in the 4^th,5^th and 6^th days?P<0.01?.Experiment 2:This experiment used the nylon bag method to objectively reflect the degradation rate of hydroponic barley grass at different time points in the rumen of dairy cows at specific time intervals.The results showed that:1)The degradation rate of CP in the 7^th day and in the rumen at 6 h was 72.08%,which was significantly lower than that in the 5^th day?P<0.05?,but not significantly different from the 4^th and 6^thh days?P>0.05?.The degradation rate of NDF in the 7^th day and in the rumen at 6 h was dramatically significantly lower than that on the 4^th and 5^th day?P<0.01?,but the difference was not significant?P>0.05?.The degradation rate of ADF in the 7^th day and in the rumen at 12 h and 24 h was significantly lower than the 4^th,5^th and 6^th days?P<0.05?.The degradation rate of ADF in the rumen at 12 h and 24 h was significantly lower than that the 4^th,5^th and 6^th days?P<0.05?.The degradation rate of ADF in the rumen at 48 h was significantly higher than that in the 4^th day?P<0.05?,but not significantly different from the 5^th and 6^th days?P>0.05?.2)The degradation parameters a and ED of DM in the rumen and in the 7^th day were dramatically significantly lower than in the 4^th,5^th and 6^th days?P<0.01?,and the degradation parameter b was dramatically significantly higher than the 4^th and 5^th days?P<0.01?,but not significantly different from the 6^th days?P>0.05?.The degradation parameter b of CP in the rumen in the 7^th day was significantly lower than the 4^th days?P<0.05?,but not significant different from the 5^th and 6^th days?P>0.05?.The degradation parameter b of NDF in the rumen was dramatically significantly higher than the 4^thh day?P<0.01?,but not significant different from the 5^th and 6^th days?P>0.05?.The degradation parameter b of ADF in the rumen was dramatically significantly lower than the 6^th day?P<0.01?,but not significant different from the 4^th and 5^th days?P>0.05?.The degradation parameter ED of ADF in the rumen was significantly higher than the 4^th and 5^th days?P<0.05?,but not significant the difference from the 6^th days?P>0.05?.Experiment 3:In this experiment,the gas production and nutrient degradation rate of barley grass in different hydroponic time were determined by in vitro gas production method.The results showed that:1)The degradation rate of DM,ME and NE_L of the in vitro fermentation at 30 h in the 7^th day were significantly lower than the 4^th,5^th and6^th days?P<0.01?.The degradation rate of NDF and ADF of the in vitro fermentation in the 7^th day were dramatically significantly higher than the 4^th,5^th and 6^th days?P<0.01?.The degradation rate of DM,ME and NE_L of the in vitro fermentation at 48h in the 7^th day were significantly lower than the 4^th and 5^th days?P<0.01?,but not significant the difference from the 6^th days?P>0.05?.The degradation rate of NDF and ADF of the in vitro fermentation at 48 h in the 7^th day was dramatically significantly higher than the 4^th,5^th and 6^th days?P<0.01?.2)The gas production parameter C of in vitro fermentation at 30 h and 48 h in 7^th day was significantly lower than the 4^th day?P<0.05?,but not significantly different from the 5^th and 6^th days?P>0.05?.The gas production parameter RmaxS of in vitro fermentation at 30 h in 7^th day was dramatically significantly higher than the 4^th,5^th and 6^th days?P<0.01?.The gas production parameter RmaxS of in vitro fermentation at 48 h in 7^th day was dramatically significantly higher than that the 4^th and 5^th days?P<0.01?,but was not significant different from the 6^th day?P>0.05?.In conclusion,the barley grass have different nutritional values during the short interval of hydroponic culture.The degradation rate of various nutrients in barley grass is faster in the rumen which is easier to be digested by cows.In the 7^th day,hydroponic barley grass have higher nutritional value and could be high quality forage grass resources available for development and usage for herbivores.