Resistance Of Different Maize Varieties To Six Fusarium In Guangxi

Maize is one of the most important food crops in China,and its planting area in Guangxi is second only to rice.Because of the special planting structure in Guangxi,maize planting areas are relatively scattered,often adjacent to sugarcane,banana and other crop planting areas.Fusarium are the main pathogens of sugarcane pokkah boeng and banana wilt and Fusarium can also cause some maize diseases.In order to explore whether the Fusarium isolated from different crops will affect maize and the difference of characteristics of different Fusarium strains,the Fusarium isolated from maize,sugarcane and banana were F.graminearum(PH-1),F.kyushuense(S5),F.verticillioides(CNO-1),F.proliferatum(YN41),F.oxysporum(BS2-6),F.oxysporum f.sp.Cubense(FOC4)and seven maize varieties commonly cultivated in Guangxi production:GD0810,GD0811,GD1125,GD901,GD162,GD165 and GD166 were studied.This study analyzed the identification and classification of different Fusarium,the pathogenicity of Fusarium and the resistance level of different maize varieties,the variety of toxicants in Fusarium and its maize ear rot,and the control effect of different medicines on Fusarium strains.The main results are as follows:(1)Six Fusarium species were identified and classified by morphological and molecular biology methods.According to the 3 gene of ITS,mtSSU and EF-1 alpha,the molecular phylogenetic tree of Fusarium spp.was made,and the classification of each strain was clarified.Two pairs of specific quantitative Real-time PCR primers were designed according to the difference of ITS sequences between PH-1(F.graminearum)and S5(F.kyushuense).The F.graminearum and F.kyushuense were successfully identified.(2)The optimum temperature for growth of six Fusarium.spp.The optimum growth temperatures of PH-1,S5 and BS2-6 were 24 ?,and those of CNO-1,YN41 and FOC4 were 28 ?.Except that PH-1 did not grow at 36 ?,the other five strains could grow at 16-36 ?.(3)Six Fusarium spp.could cause disease in leaves and ears of seven maize varieties.The order of pathogenicity of the strains to maize leaves was BS2-6>CNO-1>YN41>PH-1>S5>FOC4,and the order of pathogenicity to maize ears was S5>PH-1>CNO-1>BS2-6>YN41>FOC4.The order of maize leaves resistance to Fusarium was GD165>GD166>GD162>GD1125= GD0811>GD0810>GD901.The order of maize ears resistance to Fusarium was GD165>GD162>GD166>GD0811>GD1125>GD0810>GD901.(4)UHPLC-MS/MS was used to detect Fusarium spp.and the kinds of poisons caused by maize ear rot.PH-1 and its maize ear rot can detect ZEA,DON and 3Ac-DON toxins.S5 and its maize ear rot can detect 3Ac-DON toxins.CNO-1 and its maize ear rot can detect 3Ac-DON and FUS ·YN41 can detect FUS,FB1 and FB2 toxins,its maize ear rot can detect FUS and 3Ac-DON toxins.BS2-6 and FOC4 can detect FUS and 3Ac-DON toxins,their ear rot can detect only FUS toxins.(5)Five common fungicides were selected to screen six Fusarium strains for drug control.Carbendazim had the best inhibiting effect on six strains,followed by thiophanate methyl and manganese zinc.Etheromycin ester and cycloamycin had the worst inhibiting effect on six strains.