Effects Of EP₂ Receptor On Escherichia Coli-infected Endometrial Tissue Damage Of Dairy Cows

Endometritis of dairy cows is a common postpartum disease,which seriously restricts the development of dairy farming.Studies have shown that PGE2 plays an important role in regulating endometrial inflammation induced by E.coli in dairy cows,but its mechanism is still unclear.In order to fully understand the mechanism of action of PGE2 in promoting inflammation in E.coli-infected endometrial tissues of dairy cows,the EP2 receptor agonist and COX-2 and mPGES-1 inhibitors were used to induce endometrial tissues cultured in vitro,and the exression of PGE2 synthase(COX-2 and mPGES-1)?inflammatory factors(IL-1?,IL-6,IL-8 and TNF-a)and damage-associated molecular patterns(HMGB-1 and HABP1)was examined by RT-PCR,Western Blot,Elisa,the distribution of HMGB-1 and HABP1 in endometrial tissues was examined by immunofluorescence,and the pathological change of endometrial tissues was observed by paraffin-embedded H&E staining.The results showed that EP2 receptor agonists significantly up-regulated the expression of COX-2 and mPGES-1?inflammatory factors IL-1?,IL-6,IL-8 and TNF-a,and damage-associated molecular patterns HMGB-1 and HABP1 in E.coli-infected endometrial tissues of dairy cows,however,which was reduced by COX-2 and mPGES-1 inhibitors.Besides,Histopathological examination indicated that the endometrial epithelial cells completely detached and the gland cells disintegrated and necrosis in the EP2 receptor agonist treatment group,indicating PGE2-EP2 pathway mediates the synthesis of PGE2 in E.coli-infected endometrial tissues and promotes the expression of inflammatory factors and damage-associated molecular patterns,further result in tissue damage.