Effects Of TLR2/4 Receptor Pathway On PGE₂ Synthesis And Tissue Damage In Bovine Endometrial Tissues

Prostaglandin E2(PGE2),a lipid mediator,is released by several cell types including endometrial cells and plays a significant role during endometritis caused by bacterial infection.Recent researches have shown that PGE2 is accumulated and promotes tissue damage in Escherichia coli(E.coli)-infected bovine endometrial tissues,however,the mechanisms of which is not fully understand.This study was to investigate whether Effects of TLR2/4 receptor pathway on PGE2 synthesis and tissue damage in bovine endometrial tissues.The bovine endometrial tissues were infected with E.coli in vitro,and TLR2/4 agonists,TLR4?MyD88 and p38 MAPK inhibitors were added.The expressions of COX-2?mPGES-1?HABP1?HMCGB-1 and the secretion of PGE2 in bovine endometrial tissues were detected by fluorescence quantitative PCR,Western Blot and ELISA.The results prove that E.coli and Toll-like receptors(TLR)2/4 agonists significantly induced COX-2?mPGES-1 expression and PGE2 synthesis in bovine endometrial tissue,Similarly,they also significantly increased the expression of damage-associated molecular patterns(HMGB-1 and HABP1)and promoted tissue damage,but which was dramatically decreased by TLR4?MyD88 and p38 MAPK inhibitors in E.coli-infected endometrial tissues.Indicating TLR2/4 receptor pathway accumulates PGE2 in bovine endometrial tissue and causes damage to bovine endometrial tissue.