A Study On Primary Culture Technique Of Tissue Culture Of Acer Ginnala

In recent years,Acer ginnala has received great attention because of its beautiful tree-shaped and garden appreciation value.In conventional breeding methods of Acer ginnala,such as sowing seedlings,grafting seedlings which are susceptible to seasonal and environmental conditions.Therefore,they have become a bottleneck limiting the rapid propagation of Acer ginnala.Tissue culture technology has the advantages of being aseptic,efficient and rapid,and is of great significance for the development and utilization of Acer ginnala.This experiment is mainly to study the primary technology of tissue culture of Acerginnala,including disinfection and sterilization methods,explant selection,medium type,sucrose,pH concentration,different hormone concentration ratio,different antioxidant measures.The different hormone concentration ratios were designed by orthogonal test.The other experiments were carried out by single factor completely randomized trial design.The first-generation technical test materials for tissue culture were selected from the tea leaves maple planted from the nursery of Jiaohe City,Jilin Province,to Dadongliu Nursery,Beijing.The results are as follows:1.Studying different types of explants found that the induction of young shoots in the same year was better than that of petiole and leaves.The pollution rate of young stems was 22.3%,and the mortality rate was 14.4%,which was lower than the other two explants,and the survival rate was 63.3%.2.The young stem segments were sterilized with 0.1%HgC12 solution for 6 min,which was the best.When the primary cultured sterile seedlings were induced,the induction rate of MS medium was 62.7%,which was better than that of 1/2MS and WPM,and the pollution rate and browning rate were lower.3.The young stem segments of Acer ginnala of the Dadongliu nursery in Beijing were introduced as experimental materials.The best time for collection was early June.The young stems of this period had strong vitality,low pollution rate and browning degree less than 7-August.4.Adding 2%sucrose to the induction medium,the pH was between 5.6-5.8 had the best culture effect which will help to reduce the browning phenomenon in the primary culture process.5.In the primary culture,the hormone that plays a leading role in the young stem segment is 6-BA,and the hormone concentration combination:6-BA 0.3 mg/L+NAA 0.4 mg/L,the induction rate can reach 85%,reaching the highest level.