Study On The Therapeutic Effect Of The Extract Of Physalis Calyx Seu Fructus On DSS-induced Colitis In Mice

Objective: We have assessed the therapeutic effect of the extract from Physalis Calyx Seu Fructus on DSS-induced ulcerative colitis(UC)in mice,The aim is to study the anti-inflammatory effects of the extract from Physalis Calyx Seu Fructus.Methods: 108 Kunming mice were randomly divided into blank control group(18 rats)and DSS model group(90 rats).The blank control group mice were free to drink distilled water for 7 consecutive days.The DSS model group mice were allowed to drink 3% DSS for 7 consecutive days DSS.After establishing the colitis model,the model groups were randomly divided into DSS model control group,JDL-TQW low-dose group(0.25g/kg BW),JDL-TQW medium-dose group(0.5g/kg BW),JDL-TQW high-dose group(1g/kg BW)and salazosulfa Pyridine Positive drug control group(100mg/kg BW),with 18 rats in each group.JDL-TQW was prepared with sodium carboxymethyl cellulose as suspension and sulfasalazine as distilled water.Each group was given 0.2ml of distilled water for 7 consecutive days.The blank control group and DSS model control group were given equal volume distilled water for 7 consecutive days.From the beginning of the modeling to the end of the administration,the disease activity index(DAI)of the mice was observed according to the daily changes in body weight loss,diarrhea,fecal traits and blood in the stool;After 3d,5d and 7d,the mice were sacrificed by cervical dislocation,the whole colon tissue was taken out,the degree of colonic mucosa congestion was observed,the length of the colon was weighed,the weight of the spleen was weighed,and the colon mucosal damage index(CMDI)was evaluated.Picric acid fixed a section of colon tissue,dehydrated and embedded,HE stained,and observed the Pathological changes of colon tissue sections under the microscope;10% homogenate of colon tissue was prepared to detect the activities of SOD,CAT,GSH-Px,and QRT-q PCR was used to detect the expression levels of Proinflammatory factors IL-6 and IL-1beta in colon tissue.Results: 1.During the modeling period,the mice in the DSS model group had a marked loss of appetite,appetite and weight,no formation of feces,severe blood in the stool,and disordered hair.Compared with the blank control group,the DAI score and CMDI score of the DSS model group were significantly higher,with a significant difference(P<0.01).During the treatment period,compared with the DSS model control group,the DAI and CMDI scores of the JDL-TQW low,medium and high dose groups were not significantly different(P>0.05);the DAI score of the sulfasalazine group decreased significantly and was significant.There was no significant difference in the difference of CMDI scores(P<0.05).After HE staining,the pathological changes of colon tissue were observed.JDL-TQW can reduce the infiltration of inflammatory cells,mucosal congestion and hemorrhage.2.The body weight of the mice was weighed daily from the start of the modeling to the end of the administration.During the administration period,the weight of each group of mice was weighed after 3 days,5 days,and 7 days of administration,and the body weight of the mice was dynamically monitored.Compared with the blank control group,the mice in the model group began to lose weight on the second day,and the weight loss was most obvious from the fourth day to the seventh day.Compared with the model control group,the body weight of the mice in each of the administration groups increased,and the mice in the JDL-TQW high-dose group recovered to the initial level of the model after 7 days of administration.3.The activities of SOD,CAT and GSH-Px in the colon tissue of mice were detected at 3d,5d and 7d,respectively.Compared with the blank control group,there was a significant difference in the SOD activity of the DSS model group(P<0.05).There was a significant difference in the decrease of CAT and GSH-Px activity(P<0.01).Compared with the DSS model control group,there was no significant difference in the activity of SOD,CAT and GSH-Px in the low and middle dose groups of JDL-TQW(P>0.05).The activity of SOD and CAT increased in the high dose group of JDL-TQW for 7 days.There was a significant difference(P<0.01)on the 5th day,the activity of SOD increased significantly(P<0.01),and the activity of SOD,CAT and GSH-Px increased significantly(P<0.05).There was a significant difference in the activity of SOD,CAT and GSH-Px in the sulfasalazine group at 3d,5d and 7d(P<0.01).4.This experiment continued to use RT-q PCR to detect the expression levels of inflammatory factors IL-6 and IL-1β mRNA in DSS-induced mouse colitis tissues.The experimental results showed that compared with the blank control,the expression levels of IL-6 and IL-1β mRNA in the DSS model control group were significantly increased(P<0.001).Compared with the DSS model control group,the low dose of JDL-TQW decreased the expression levels of IL-6 and IL-1β mRNA at the 3rd and 5th day of treatment,and there was a significant difference at the 7th day(P<0.05);JDL-TQW The expression levels of IL-6 and IL-1β mRNA were significantly decreased in the middle and high dose groups at 3d,5d and 7d,and there was a significant difference(P<0.01).Conclusion: 1.JDL-TQW can improve the fecal traits of mice with colitis,relieve the weight loss of mice,and reduce the disease activity index(DAI)and mucosal injury index(CMDI)of mice,indicating that JDL-TQW is small for 3.0% DSS induction.Murine colitis has a certain relief effect.2.JDL-TQW can increase the antioxidant activity of SOD,CAT and GSH-Px in colon tissue of model mice.These results suggest that JDL-TQW may alleviate DSS-induced colitis through the antioxidant function of the body,thus Playing an anti-inflammatory role.3.JDL-TQW can inhibit the expression of Pro-inflammatory factors IL-6 and IL-1beta in colon tissue of mice,thereby reducing the secretion of inflammatory factors and achieving the effect of treating inflammation.