Effects Of Maternal Yeast-based Nucleotide Supplementation On Performance Of Sow And Intestinal Development Of Neonatal Piglets

The high mortality rate of neonatal piglets was one of the key factors limiting the production performance of sows.The diarrhea and even death caused by the incomplete development of the intestinal structure and development of neonatal piglets were the main reasons.This study was conducted to investigate effects of yeast nucleotides on the production performance of sow and intestinal villus morphology of neonatal piglets.Sixty pregnant sows(day 80 ± 5)were assigned to two groups based on parity: the CON group(fed a basal diet)and YN group(fed a basal diet with 3 g YN / kg diet).The experiment was started from the 85 th day after sow's pregnancy to the end of the weaning of piglets(20 days).The reproduction performance of sows,diarrhea rate and growth performance of piglets were recorded.In addition,plasma of sows were taken during sow delivery,plasma?liver and intestinal samples of neonatal piglet were obtained immediately after birth.The plasma biochemical parameters of neonatal piglets were detected by biochemical kits.The intestinal morphology of neonatal piglets was observed by the HE staining.The mRNA expression level of intestinal tight junction protein and cytokine in neonatal piglets were detected by quantitative real time-polymerase chain reaction(RT-PCR)The results were showed as follows: 1)Compared with the control group,nucleotides reduced significantly the sow's delivery time,the number of stillbirths,and the number of IUGR(P<0.05).At the same time,the weaning weight of piglets in increased significantly(P<0.05).In addition,nucleotides had a tendency to increase the birth weight of neonatal piglets(0.05<P<0.1);Compared with the control group,maternal yeast-based nucleotides supplementation significantly reduced the diarrhea rate of suckling piglets(P<0.001);2)Compared with the control group,maternal yeast-based nucleotide supplementation significantly increased serum ALB,HDL and IgG of sow(P<0.05).In addition,the plasma LDL of sows in YN group was significantly reduced(P<0.001);Compared with the control group,maternal yeast-based nucleotide supplementation significantly increased the plasma CHO,HDL and LDL in neonatal piglets(P<0.05).In addition,the nucleotides had a tendency to increase plasma TP in neonatal piglets(0.05<P<0.1);3)Compared with the control group,maternal yeast-based nucleotides supplementation significantly increased the SFA,C18?0 and C20:4N6 in liver of neonatal piglets(P<0.05).In addition,nucleotides significantly decreased MUFA,C20:0,C18?1N9C and c11 C20:1 in liver(P<0.05),the nucleotides had a tendency to increase C18:3N3 in liver of neonatal piglets(0.05<P<0.1);4)Compared with the control group,maternal yeast-based nucleotides supplementation significantly increased liver Met,Lys and Tyr of neonatal piglets(P<0.05).At the same time,nucleotides had a tendency to increase liver the total amount of EAAs and NEAAs,Thr,Val,Leu,Ile,Phe,Arg,Glu,Gly(0.05<P<0.1);5)Compared with the control group,maternal yeast-based nucleotides supplementation significantly increased the VH and V/C of duodenum in neonatal piglets;In addition,the CD of duodenum was reduced in YN group(P<0.05);6)Compared with the control group,maternal yeast-based nucleotides supplementation increased significantly mRNA expression of ZO-1,NF-?B,and TLR4 in ileum of neonatal piglets(P<0.05).In addition,nucleotides have a tendency to increase mRNA expression of duodenal IL-1? and ileac INF-?(0.05<P<0.10).Over all,maternal yeast-based nucleotides supplementation decreased the stillbirth rate,IUGR and diarrhea rate of suckling piglets,and promoted intestinal villus development and mRNA expression of intestinal cytokines to a certain extent,which is beneficial to improve the productive performance of sow.