Genetic Structure And Geographic System Of Geographical Population Of Pinus Tabuliformis Mountain Range In Shanxi Province Using SSR Markers

Pinus tabuliformis Carr.of the subgenus Diploxylon(Pinaceae)is a major and widespread component of coniferous forests in northern China.Shanxi province is the center of the distribution of P.tabuliformis in China.It covers the central and northern regions of P.tabuliformis seed region,and is also an important provenance for cultivation of P.tabuliformis plantation in surrounding provinces.According to the survey,the seeds of P.tabuliformis plantation constructed in Beijing,Hebei,Liao Ning and other places from 1930s to 1980s are mostly from Shanxi Province,but the specific seed sources are unclear.The results showed that the adaptability and growth performance of different geographic provenances of P.tabuliformis were significantly different between Hebei and Liaoning,and the seed source selection of P.tabuliformis was very important to the quality of plantation.The Analysis of genetic structure of P.tabuliformis provenance can provide a theoretical basis for the exploration of the origin and trace to the source of P.tabuliforlis Artificial Forest.In this study,eight pairs of specific SSR markers and four pairs of differentially significant primers of P.tabuliformis were used to analyze multi-population with the population genetic method.The study is devoted to resolve the molecular variation and genetic diversity in the five mountains of Shsanxi province,analysis the genetic structure of the geographical population,clarify the geographical system between the populations,and explore the possible relations between influencing factors and forest growth.The results of the study show that:(1)The results of genetic diversity using eight pairs of primers showed that the genetic variation of Guandi Mountain was abundant and Zhongtiao Mountain was lower.Four pairs of primers showed that Guandi Mountain and Taihang Mountain had abundant variation,and there was a lower variation in Zhongtiao Mountain.Four pairs of basic primers can get similar results to eight pairs of primers,and most of the parameters are higher than eight pairs of primers.(2)Eight pairs of primer analysis showed that the differentiation of five mountains was small(FST=0.032 8)and four pairs of primers analysis showed that there was a moderate degree of differentiation in five mountain populations(FST=0.051 9).Four pairs of basic primers showed that the more significant differentiation,and the rest results were similar to eight pairs of primers.(3)There was a significant correlation between the genetic distance and geographical distance of the five mountains population.eight pairs of primers showed a significant correlation between genetic distance and geographical distance(r=0.69442,p=0.9448),and four pairs of diffberentially significant primer analysis showed a significant correlation between genetic distance and geographical distance(r=0.70447,p=0.9470).Four pairs of basic primers with significant difference could obtain the same genetic difference detection effect as eight pairs of primers.(4)The genetic diversity parameters and temperature in January had a negative correlation trend.The ratio of annual precipitation and temperature in January and parameters of genetic diversity have positive correlation.The study reveals that there are relatively small genetic differentiation and rich genetic diversity among the geographical populations of P.tabuliformis in the main mountains of Shanxi province.SSR markers can effectively display the genetic structure characteristics of various populations,and the limited difference marked markers can be more effectively applied to the population genetic analysis of P.tabuliformis.The relative level of water and heat of habitat meteorological factors has a dominant influence on the genetic diversity of the population,and a relatively high ratio of water and heat is conducive to maintaining a higher genetic diversity of the population.