The Genetic Diversity Analysis Of Different Roegneria Ciliaris Populations

Roegneria ciliaris (Trin.), belonging to annual herb Roegneria, is a wild relativeof wheat and provides a wealth of wild genetic resources for the cultivation of newwheat varieties. Accumulating evidence has been demonstrated referring to thedisease resistance, breeding and isozyme in R. ciliaris, whereas limited research hasbeen reported in the genetic diversity. Therefore, by using three molecular markingtechniques SRAP (sequence-related amplified polymorphism), SSR (simple sequencerepeat) and ISSR (inter simple sequence repeat), we analyzed the gene diversity of 36R.ciliaris populations in Huang-Huai-Hai area, a main wheat producing area in China,and further outlined the relationship between the genetic distance of R. ciliarispopulations and the eco-geographic locations.The main results are as following:1. Using SRAP, we outlined the genetic diversity levels of 36 R. ciliarispopulations. The results show that based on the mean value of GS 0.77, the 36 R.ciliaris populations may be divided into 7 groups which are Zhengzhou groups,Suqiangroups,Xinyang groups,Yantai groups,Jinan groups,Binzhou groups,Binzhougroups,Qingdao groups; Specifically, the value of polymorphism percentage points(P), Shannon diversity index (I) and Nei genetic diversity index (H) are 39.78%,0.2331 and 0.1600; Genetic distance (GD) are among 0.1370 and 0.3766；In theAMOVA study, the mean value of Fst and Nm is 0.3351,0.5551 respectively.2. The results of SSR show that based on the mean value of GS 0.74, the 36 R.ciliaris populations may be divided into 7 groups which are Binzhou groups,Dezhougroups,Qingdao groups,Jining groups,Jinan groups,Xinyang groups,Zhengzhougroups. The values of P, I and H are 39.78%, 0.2331 and 0.1600 according to SSRanalysis;GD are among 0.1112 and 0.4648;In the AMOVA study, the mean value of Fst and Nm is 0.3442,0.4221 respectively.3. The results of ISSR show that based on the mean value of GS 0.72，the 36 R.ciliaris populations may be divided into 5 groups which are Lianyunganggroups,Binzhou groups,Taian groups,Zhengzhou groups,Weifang groups. The valuesof P, I and H are 36.19%, 0.1873 and 0.1253 in ISSR analysis. GD are among 0.0581and 0.5346; The mean value of Fst and Nm is 0.4355,0.3114 respectively with theAMOVA analysis.4. We uses these three methods to divide the 36 R. ciliaris populations intodifferent ecological groups. Although the ecological groups has different memmbers,it also tell us some trend that the populations which have closer latitude prioritytogether first. The diversity populations centralized in Heze population,Jiningpopulation,Taian population,Dezhou population,Weifang population. The values of P,I and H obtained from different molecular markers are in great coincidence, reflectinga high level of genetic diversity in R. ciliaris groups.And the values of Nm are all lessthan 1,the Fst values are all above 0.1500 suggesting that it is the differences of thelocation that accounted for the low frequent gene communication and high frequentgenetic structure diversification in R. ciliaris groups.5. The relationship between the eco-geographical differences and the geneticdiversity of R. ciliaris groups was evaluated using Mentel test. Comparing thegenentic distance metrix with geographical distance metrix, the correlation value (r) is0.3273 in SRAP, 0.3110 in SSR study and -0.379 in ISSR study. The SRAP, SSRanalysis showed that the genetic distance of R. ciliaris groups is positive related withthe geographic location, where a larger the genetic distance indicated a larger geneticdiversity. In contrast, ISSR analysis indicated that the genetic distance of the R.ciliaris groups and geographic location was negatively correlated, indicating thatthere are lots factors that are responsible for the genetic differences of the R. ciliarisgroups.