Isolation,Purification And Identification Of 11 Species Of Accompanying Fungi With Xerocomus Spadiceus

Xerocomus spadiceus,belongs to Basidiomycota,is one of the most precious wild edible mushroom in Yunnan Province.Xerocomus spadiceus is widely distributed in Yunnan Province and of high economic value.The resources have been affected by various factors in recent years,so it's difficult to be sustainable utilized.As one of mycorrhizal fungi,Xerocomus spadiceus cannot be cultured artificially.The growth mechanism of Xerocomus spadiceus under natural conditions is unknown and when pure cultured it just grows slowly.At present,studies mainly focuses on classification,artificial fermentation of mycelia and so on.It is difficult for production application.Soil in natural habitat were collected and 11 accompanying fungi were isolated and identified morphologically and molecularly by ITS sequencing.Under natural conditions,accompanying fungi may promote the formation of fruiting bodies of large fungi.The purpose of this paper is to screen these associated fiungi and lay a theoretical foundation for the artificial culture of Xerocomus spadiceus.The main study results are as follows:1.Eleven fungi were isolated,which named as S1,S3,S4,S6,S18,S26,S30,S31,S47,S62,S65,and their ITS sequence length are 606bp,588bp,586bp,627bp,627bp,609bp,666bp,579bp,675bp,606bp,569bp.2.By morphological characteristics comparing and ITS sequences comparing in NCBI database,preliminary determination sequences and their similarity are as follows:S1 was Paecilomyces carcass(99%),S3 was Verticillium fungicola(99%),S4 was Penicillium chalabudae(99%),S6 was Trichoderma ovalisporum(99%),S18 was Arthrinium marii(99%),S26 was Pochonia bulbillosa(99%)and S30 was Umbelopsis dimorpha(100%).S31 was Fusarium solani(100%),S47 was Metarhizium flavoviride(96%),S62 was Pestalotiopsis disseminata(99%)and S65 was Fusarium graminearum(100%).3.The phylogenetic tree of 11 fungi,X.spadiceus and B.aereus was build and the result showed that S1(Paecilomyces carcass),S3(Verticillium fungicola),S4(Penicillium chalabudae),S18(Arthrinium marii),S26(Pochonia bulbillosa),S31(Fusarium solani),S47(Metarhiziumflavoviride),S62(Pestalotiopsis disseminata)and S65(Fusarium graminearum)are closely related to Boletus.4.4 species of accompanying fungi were dominant fungi in associated community,which are Paecilomyces carcass,Verticillium fungicola,Penicillium chalabudae and Pochonia bulbillosa.