The Effect Of Angelica Sinensis Polysaccharide On Head Kidney Leukocytes Immunity?Immune Related Genes Expression And Protein Expression Of Epinephelus Malabaricus

In this experiment,Epinephelus malabaricus is experiment objective;by the way of incubation in vitro of Angelica sinensis polysaccharide,to test immunocompetence of head kidney leukocytes,gene expression of TLR22 related pathways,impact of protein expression.Thirty healthy Epinephelus malabaricus divided into three groups randomly.And head kidney leukocytes isolated from head kidney which removed from Epinephelus malabaricus.Head kidney leucocytes were cultured in vitro with ASP(200,400,800,1600,3200,6400 ?g/m L).Leucocytes proliferation,respiratory burst and bactericidal capacity were assayed after incubation.Results showed that the proliferation,respiratory burst activity and bactericidal capacity of leucocytes incubated ASP was boosted.Thirty healthy Epinephelus malabaricus divided into three groups randomly.And head kidney leukocytes isolated from head kidney which removed from Epinephelus malabaricus.To investigate effects of ASP on the expression of TLR22 signal pathway-related genes in E.malabaricus,head kidney leucocytes of E.malabaricus were cultured with ASP in vitro for different time(3h,6h,12 h,24h,and 48h),and changes in m RNA levels of TLR22 pathway-related genes were quantified using QPCR technology.Results showed that CASP,ASP were significantly affect the point grouper TLR22 amount on the expression of related genes and pathways,and with the extension of incubation time,the amount of gene expression have significant changes.Forty five healthy Epinephelus malabaricus divided into three groups randomly.And head kidney leukocytes isolated from head kidney which removed from Epinephelus malabaricus.With incubation concentration of 800 ?g/m L of CASP incubation grouper kidney cells for 24 h,fish head with i TRAQ technology were used to detect angelica polysaccharide of fish head with protein expression in kidney of white blood cells,In this project,a total of 1301 proteins were identified,of which,916 proteins contain at least two unique peptides.There were 63 proteins differentially expressed(p?0.05 and more than 1.2-fold change)in sample 24 C compared to 24 D.Besides,all the identified proteins were subjected to bioinformatic analysis by using the Gene Ontology(GO)database,the Kyoto Encyclopedia of Genes Genomes(KEGG)database and the Cluster of Orthologous Groups of proteins(COG)database to find the relative processes that these proteins were involved in.Furthermore,function enrichment analysis(GO enrichment and KEGG enrichment)of these differentially expressed proteins showed enrichment in several biological pathways which can characterize the most significant functional roles and related pathways...