Isolation,Identification And Antibacterial Activity Of Cellulase Producing Strain

The cellulase producing strain ZL-16 was isolated from the soil,whice used the method of six sampling to get the sail from Panquangou,In this study,I get 19 high cellulase production strain by the separation method of the Congo red.The standard of separate bactera is that if the diameter of transparent ring ratio the Colony diameter exceed 3,this production srain is well.ZL-16 is the most productive srain in the 19 high cellulase production strain which its ratio is 9.94 the ZL-16 strains,according to the morphological identification,physiological,biochemical and molecular identification,which belongs to Bacillus amyloliquefaciens.Before to study ZL-16 how to agaist the Phytophthora capsici,I must to identify ZL-16 is not ifuence on plant’s cell wall.To identify its influence low to the plant,we can use the DNS and conductivity method to musure the data.In this study,I processes the plant’s leaves and roots by the crude enzyme solution and broth.The result show that crude enzyme solution and ferment solution.have no influence an the leaves an roots.Crude enzyme solution’s injure rate to the insolate leaves is 5.2%,to the leaves is 0.8%.Ferment solution’s injure rate to the insolate leaves is 4.9%,to the leaves is 0.8%.Crude enzyme solution’s injure rate to the insolate roots is 2.1%,to the roots is 0.4%.Ferment solution’s injure rate to the insolate roots is 2.0%,to the roots is 0.39%.All in all,ZL-14 has less ifluence on plants.To design the fermentation conditions of ZL-16,I need to use the method of single factor and orthogonal,Finally,I determine the best of nutritional conditions about ZL-16 is thar:Peptone 1.7g,CMC-Na8g,FeSO4 · 7H2O 0.15g,ZnS04 0.35g,K2HP044.5g,1000ml of distilled water.The optimum environmental conditions is that liquid volume 30ml,inoculum size 2ml,pH8,rotation speeed 160/min,temperature 34 ℃.To study antibacterial activity of cellulase producing strain to Phytophthora capsici.I use The method of tablet confrontation,micrography,electric mirror etc.Through a series of experiments,we can gen a conclusion that ZL-16 has good effect to Phytophthora capsici.