The Microbial Diversity And The Response To Inoculant Of Corn Silage

In order to study the effects of different microbial inoculum on the fermentation and aerobic stability of corn silage,the microbial diversity of corn silage was analyzed by two methods,the traditional culture method and the high-throughput sequencing.In Expt.l,The microbial diversity of the whole corn silage in 5 different areas were studied and compared.Traditional culture can’t determine microbial diversity of whole crop silage accurately,although high-throughput sequencing also did not identificate the microorganism of whole corn silage at species level,it can be a much more comprehensive response to the microbial abundance of whole corn silage at genus level,So as to reveal the whole corn silage microbial diversity of the foundation.In Expt.2,The Lactobacillus buchneri were screened from the whole corn silage,the strain producing acid performance was good,pH value has reached 4 in 10 h.Enter the stable period in 14 h,then the changes of OD600 value were relatively small.At pH 3 or 3.5,it was barely growth.The acid resistance was not very good.The Lactobacillus buchneri grew well in 25～60 ℃,resistant of low and high temperature was well.Lactobacillus buchneri was made into freeze-dried inoculant.In Expt.3,Sterilized or no sterilized,the whole corn was respectively added in Lactobacillus plantarum,Lactobacillus buchneri,Pediococcus ethanolidurans,Clostridium butyricum,Lactobacillus rhamnosus,Escherichia coli.The influence of 6 different additives on silage fermentation quality and nutrients was evaluated.Addition of Lactobacillus buchneri can significantly reduce dry matter preserved rate(P<0.01).The LA and AA content of adding 6 different additives of whole corn silage were significant difference(P<0.01).Silage of adding Lactobacillus buchneri had best aerobic stability.