| Since the 20th century,the United States,Netherlands and other countrieshave made a lot of work on collection of Hemerocallisspp.accessions,cross-breeding,etc..The statistics of American Hemerocallis Society showed that the quantity of Hemerocallis varieties logged currently has been over 82,125.Our country is the distributioncenter of Hemerocallis genus in the world.For a long time,the collation and utilization of Hemerocallis genus resources was relatively backward.In order to change this situation and provide a basis for creation of new excellent germplasm about Hemerocallisspp.,we studied 155Hemerocallis germplasm which were collected from home and abroad.85 samples of 155Hemerocallis germplasm was usedto record 13 traits about flower.We analyzed the genetic structure of 155 Hemerocallisspp.accessions by 396 variation sites which was obtained from the result of PAGE testing on PCR products of 155 Hemerocallis germplasm based on 43 pairs of EST-SSR primers.1.The finding of 13 phenotypic traits of 85 test materialsobserved showed thatphenotypic traits of Hemerocallis spp.accessionsis very abundant.(1)According to the Royal Horticultural Society Plant Color Chart,the inner and outer tepals of 65 samples of the test material belonged to the same color system in the color chart.The 65 test material is broadly divided into 5 groups of color system,where the yellow was the main color,including 23 materials.85 parts of the test materialsinvolved 10 different colors modes,6 flower forms and 15 inflorescence modes.Proportion of single color distribution patterns was the largest,the value was 33.00%,which mainly included H.citrinapopulation.Hom type was the main flower form,comprising 55 parts of the materials,and the proportion is 64.70%;Dichotomous branching is the main inflorescence form,including 29 materials.The number of branches of accessions flowering in the night was obviously more than those flowering during the day.Outer tepals of the test materials was mainly waterfall type,including 71 samples of materials,and inner tepals was mainly frill type,comprising 51 parts of materials.(2)Booming time of 85 materials for study was mainly two time periods,6:00?9:00 in the morning and 19:00?21:00 in theevening.The population of flowering during day mainly consisted of H.fulva population and H.'Pan long',which belonged to the H.citrinepopulation.The population of floweringin theevening mainly included H.minor,H.lilio-asphodelus,H.citrina and its local cultivars,wild species.(3)The coefficient of variation of flower diameter,length and width of the inner tepals,length and width of outer tepals,inflorescence length and the quantity of flower were respectively 20.94%,19.69%,36.09%,19.33%,36.05%,84.99%and 90.70%.The result of Seven kinds of phenotypic correlation analysis showed that inflorescence length,the quantity of flower and width of the inner tepals was significantly negative correlation(P<0.01),and width of outer tepals was significantly negative correlation(P<0.05).2.The results of the genetic structure analysis were as follows:(1)Genetic diversity analysis showed that a total of 396 alleles were obtained from 43 polymorphism SSR primers for the 155 tested materials,with an average of 9.21 alleles per SSR primers.Polymorphism information content(PIC)of primers ranged from 0.259 to 0.907,with an average of 0.606.And it showed that the selected primers could provideextensive genetic information.(2)SM(Simple matching coefficients of similarity)among varieties were calculated byNTSYS software showed that similarity coefficients ranged from 0.770 to 1.000,with anaverage of 0.859.Similarity coefficient of 31.82%of accessions concentrated between 0.855 to 0.875.(3)Cluster analysis based on simple matching coefficients of similarityby NTSYSpc2.10 software detected two subgroups in the 155 Hemerocallis spp.cultivars(or lines),when coefficient = 0.86.Groupl and group2 included 59 and 96samplesrespectively.(4)Cluster analysis based on genetic distance by MEGA 5.05 software detected two subgroups in the 155 Hemerocallis spp.cultivars(or lines),group 1 and group2 included 70 and 85 samplesrespectively.(5)Population structure that analyzed by STRUCTURE 2.3.4 software showed that the 155 samples were divided into 2 subgroups(POP-1 and POP-2)with 55 and 100 samples,respectively.Set K=1?15,Burn-in Period parameters and MCMC was 10,000 and 100,000,respectively,and each K value was calculated for 20 repetitions.The study provided theory bases to arrange and protect Hemerocallis spp accessions,empolder reasonably,identify relationship of different Hemerocallis spp and select and breed excellent cultivars.Besides,the study made foundational preparations for association analysis of phenotypic traits of Hemerocallis germplasm using EST-SSR marker.Ultimately,the investigation would impact deeply onHemerocallis germplasm direct application in landscape. |
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