The Study Of Inducting Lycium Ruthenicum Murr.polyploid By Using Colchicine

The purpose of the experiment,using colchicine as induce reagent,was for the method of Lycium ruthenicum Murr.tetraploid induction.Through induction,got Lycium ruthenicum Murr.tetraploid successfully and concluded the optimal method.Set up the tissue culture of diploid and tetraploid rapid propagation system of Lycium ruthenicum Murr..Build the system of suitable for tissue culture,mutagenesis and evaluation technology of Lycium ruthenicum Murr.tetraploid.Simultaneously,with diploid of Lycium ruthenicum Murr.as control,studied the change of variants of the morphology and cytology.The major findings were:1 By processing seeds to induce tetraploid directly,induced mutation rate was 54.2% come to the Lycium ruthenicum Murr.tetraploid plants when the seeds treat time was 72 h,the concentration of colchicine was 0.3% and the temperature was 25?.2 The best medium for callus induction with diploid Lycium ruthenicum Murr.leaves was MS+0.125 mg·L^-16-BA+0.025 mg·L^-12,4-D,and the rate was as high as 96%;the best medium for callus induction of adventitious bud was MS+0.25 mg·^-16-BA+1mg·L^-1IAA;the best medium for the elongation of adventitious bud was MS+0.25 mg·L-16-BA+1mg·L^-1IAA;the best medium for rooting was MS.3 When cultivating the aseptic seedling of Lycium ruthenicum Murr.the condition about lowest rate of contamination and the most growth increment was 75% alcohol treatment 3 min,5% sodium hypochlorite processing time for 10 min.4 The best medium for Lycium ruthenicum Murr.tetraploid callus induction was MS +0.125mg·L^-16-BA+0.01 mg.L^-12,4-D+2%DMSO+0.01mg·L^-1colchicine.The Induction rate was 37%,and tests showed that 2% DMSO can increase the Lycium ruthenicum Murr.tetraploid mutation frequency effectively.5 The best medium for Lycium ruthenicum Murr.tetraploid callus induction of adventitious bud was MS+0.25mg·L^-16-BA+1mg·L^-1IAA,the best medium for rooting was MS.6 According to evaluation,it is easy to appear Mosaic phenomenon by seeds candidated.Mixed culture method in suitable concentrations can induce homozygous tetraploid Lycium ruthenicum Murr.With coldness and soaking method could not succeed to induced to get Lycium ruthenicum Murr.tetraploid callus.Therefore mixed culture was the optimal method to get tetraploid Lycium ruthenicum Murr..7 There were some phenomenon like slow-growing and plant-short about the tetraploid Lycium ruthenicum Murr.The modalities included that,leaves got bigger and thicker,the color of the leaves got darker,the stems got thicker,the stomatal density of leaves increased.