Effects Of Zn?Se And Chinese Herbal Extracts On Function Of Porcine Alveolar Macrophages 3D4/2

This experiment aims to explore difference of Zinc(ZnSO4/ZnGly),Selenium(Na2SeO3/Met-Se),Astragalus polysaccharide(APS),Acanthopanax senticosus saponins B(ASS),Ginsenosides F3(GS),Chlorogenic acid(CGA),and Lobetyolin(LT)and Levamisole Hydrochloride(LD)on pig macrophage immune function.This test used the different concentrations of Zn?Se and compunds respectively with normal Porcine Alveolar Macrophages 3D4/2,LPS-induced or CY-induced 3D4/2 cell in vitro,and the activity of proliferation,phagocytosis and cytokines proudcution of macrophage were measured by the methods of phagocytosis neutral red,CCK-8 and ELISA.The results were as follows:1.LPS(10 ?g/ml)increased porcine alveolar macrophages 3D4/2 phagocytosis than normal group(P<0.01),and LPS(10 ?g/ml)significantly increased the secretion of NO,TNF-?,IL-1(3,IL-10 and IL-6 in supernatant of macrophages 3D4/2 than normal group(P<0.05);CY(500 ?g/ml)cultured cell reduced significantly cell proliferation and phagocytosis(P<0.05);2.ZnSO4(10,20 ?mol/l)enhanced the ablivity of porcine alveolar macrophages 3D4/2 proliferation and phagocytosis(P<0.05),the proliferation capacity of ZnSO4(20 ?mol/l)was higher than ZnGly(P<0.01),the phagocytosis capacity of ZnGly(10,20 ?mol/l)was higher than ZnSO4(P<0.01),which reduced cell proliferation activity at 80 ?mol/l(P<0.01);ZnSO4 and ZnGly(10,20 ?mol/l)concerted with LPS enhanced the activity of proliferation and phagocytosis(P<0.05),the phagocytosis of ZnGly(10 ?mol/l)was higher than ZnSO4 group(P<0.01),and the proliferation of ZnSO4(10,20,40 ?/l)group was higher than ZnGly group(P<0.05),they reduced the proliferation and phagocytosis of activated cell at 80?mol/l(P<0.01).ZnSO4 and ZnGly(10-40 ?mol/l)increased cell proliferation inhibited by CY(P<0.05),ZnGly(40 ?mol/l)enhanced the capacity of phagocytosis to normal cell level(P<0.05).ZnSO4 and ZnGly(10,20 ?mol/l)reduced NO,TNF-?,IL-1?,IL-6 and IL-10 than LPS-induced group(P<0.05),the production of NO,IL-10 of ZnGly were less than ZnSO4 at 20?mol/l(P<0.05).3.Na2SeO3(0.5,1 ?mol/1)and Se-Met(0.1,0.5,1,5,10 ?mol/l)enhanced porcine alveolar macrophages 3D4/2 proliferation(P<0.05),and Se-Met(10 ?mol/1)was higher than Na2SeO3(P<0.01);Na2SeO3 and Se-Met enhanced porcine alveolar macrophages 3D4/2 proliferation and phagocytosis at 0.005,0.01,0.05,0.1,0.5,1 ?mol/l(P<0.05),Se-Met was higher than Na2SeO3 at those levels(P<0.05);Na2SeO3(10 pmol/l)reduced cell of phagocytosis and proliferation(P<0.05),however,Se-Met enhanced phagocytosis at the same levels(P<0.01),and SeMet(5,10 ?mol/l)was higher than Na2SeO3(P<0.05).Na2SeO3(0.5,1,5,10 ?mol/l)and Se-Met(1 ?mol/l)enhanced the activated cell proliferation than LPS-induced group,Na2SeO3(0.5,10 ?mol/l)was higher than Se-Met;Na2SeO3(0.005-10 ?mol/l)and Se-Met(0.005,0.05,0.5,5,10 ?mol/l)enhanced the phagocytosis of cell,and Na2SeO3(0.01,0.05,0.1,1,5 ?mol/l)were higher than Se-Met(P<0.05).Na2SeO3 and Met-Se(0.5-1 ?mol/l)increased cell proliferation inhibited by CY(P<0.05),Na2SeO3(0.1,0.5?mol/l)was lower than Se-Met(P<0.05);Se-Met(0.1,0.5?mol/l)enhanced the capacity of phagocytosis to nomal cell level(P<0.05),and Se-Met was significantly higher than Na2SeO3(P<0.05).Na2SeO3 and Met-Se(0.1,0.5?mol/l)reduced the secretion of NO,TNF-a,IL-1? and IL-6(P<0.05),Na2SeO3(0.5 ?pmol/1)increased the production of IL-10,and which was higher than Se-Met(P<0.05).4.APS,ASS,LT(25-200 ?g/ml),GS(25-200 ?g/ml)and LD(50,100 ?g/ml)had no difference of normal porcine alveolar macrophages 3D4/2 proliferation,CGA(25 ?g/ml)and ASS(50,200 ?g/ml)increased cell proliferation(P>0.05).ASS,APS,CGA,LT,GS and LD could enhance cell phagocytosis(P<0.05)at those levels,LD(200,400?g/ml)decreased cell proliferation and phagocytosis(P<0.05),CGA(100,200 ?g/ml)of inhibited cell proliferation,but enhanced phagocytosis of individuals(P<0.01).LT(25,50 ?g/ml),ASS,CGA(25,50 ?g/ml),GS(40 ?g/ml)and LD(100 ?g/ml)increased cell proliferation and phagocytosis(P<0.05)than LPS-induced group;LD(200?400 ?g/ml)reduced cell proliferation(P<0.05),and significantly increased phagocytosis of individuals(P<0.05).APS(25-200?g/ml)and CGA(200 ?g/ml)inhibited cell phagocytosis(P<0.05).GS(10-40?g/ml),CGA(100 ?g/ml),APS and ASS(25-100 ?g/ml)increased cell proliferation inhibited by CY(P<0.05),GS(40?g/ml),CGA(100 ?g/ml)enhanced the capacity of phagocytosis to normal cell level(P<0.05).ASS(25-50 ?g/ml)reduced the secretion of NO,IL-1? and IL-6 than LPS-induced group(P<0.05);LT(25-50 ?g/ml),CGA could reduce cell released NO,IL-1?,TNF-a,IL-6 and IL-10(P<0.05);GS(5,10 ?g/ml)reduced the production of NO,IL-1? and IL-6(P<0.05),increased the level of IL-10(P<0.05);LD(50,100 ?g/ml)reduced NO,TNF-a,IL-6(P<0.05),increased IL-10(P<0.05),and improved the ability of anti-inflammatory.These results suggested:the difference of zinc,selenium,GS,CGA,ASS,LT and LD could improve normal cells,LPS or CY-induced porcine alveolar macrophages 3D4/2 proliferation and phagocytosis,and reduce the excessive secretion of NO,TNF-a,IL-10,and IL-6 of pro-inflammatory cytokines induced by LPS,GS,LT,LD can also increase the secretion of inflammatory cytokines IL-10.In addition APS also promote cell phagocytosis,relieve the inhibited by CY and improve immune function of macrophages.