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The Use Of An Integrated Chemical-,Genetic-and Ecological-based Approach For The Quality Variation Of Traditional Chinese Medicine

Posted on:2019-01-17Degree:MasterType:Thesis
Country:ChinaCandidate:L LiFull Text:PDF
GTID:2393330572453386Subject:Pharmacy
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The quality variation of Chinese traditional medicine is the result of long-term adaptation and natural selection of species to different ecological environment.Genetic factors are the biological essence of quality variation,and environmental factors are the ecological mechanism of the quality variation.The geographical and climatic conditions of our country are complex and diverse,and the quality of the same medicinal materials from different habitats is remarkable,forming a unique phenomenon of Chinese traditional medicine.In this paper,the quality variation mechanism of Cistanche deserticola and Artemisia annua was analyzed by the new system of comprehensive analysis of chemical-genetic-ecological correlation,integrating transcriptome,metabonomics,DNA barcoding,UPLC-QTOF/MS and Geographic Information System for Global Medicinal Plants(GMPGIS).The main results are as follows:1.The primal data of 7.54G,7.50G and 7.52G were sequenced by high throughput sequencing technology for 3 different habitats Cistanche deserticola of saline alkali,grassland and desert.52043 transcripts were obtained from the transcript.The length of the transcript ranged from 201 bp to 15544bp,and the N50 length of transcript was 1362bp.Candidate genes of 11 key enzyme genes including Phenylalanine ammonia-lyase(PAL),Cinnamate 4-hydroxylase(C4H)and Cinnamate4-hydroxylas(4CL)were found.Through the detection and annotation of single nucleotide variation(SNP),36 SNP containing Unigenes annotated to phenylpropane biosynthesis pathway were found,of which 5 unigenes were PAL candidate genes and 3 unigenes were 4CL candidate genes.The differential expression and variation of these genes may be the biological reasons leading to the difference in quality of Cistanche deserticola.The suitability regionalization and ecological factors of Cistanche deserticola were studied by GMPGIS technology.It was found that China,Kazakhstan and Mongolia are potential suitable areas for the growth of Cistanche deserticola.The annual average sunshine and the relative humidity had great influence on the growth of Cistanche deserticola.In addition,the establishment of the database for DNA barcoding,transcriptome,metabonomics and ecological data of Cistanche deserticola data is conducive to data management and sharing.2.The analysis of quality variation of Artemisia annua showed that there were great differences in artemisinin content in different parts of the country.Artemisinin content of Artemisia annua in the south of Qinling Mountains Huaihe demarcation line is relatively high,and the trend of spreading around Wuling mountain area is decreasing.However,the content of artemisinin in the north of the boundary is generally low.The genetic variation of Artemisia annua was analyzed by DNA barcoding.It was found that there were significant genetic differences and rich diversity in the germplasm resources of Artemisia annua,and the genetic diversity of Artemisia annua was related to a certain region.The genetic diversity in the south of Qinling Mountains Huaihe demarcation line is particularly rich,which is similar to the distribution trend of artemisinin content.The potential distribution area of high quality Artemisia annua was predicted by GMPGIS.It was found that the south area of Qinling Mountains Huaihe demarcation line was the largest ecological suitable area for the high quality Artemisia annua.Sunshine and the relative humidity are the key ecological factors affecting the quality of Artemisia annua.The analysis results provide a new idea to better explain the formation mechanism of the quality variation of Cistanche deserticola and Artemisia annua,and provide scientific basis for the study of the theory of quality variation of Chinese traditional medicine.
Keywords/Search Tags:Quality variation of Chinese traditional medicine, transcriptome, metabonomics, Cistanche deserticola, Artemisia annua
PDF Full Text Request
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