Cloning, Expression And Immune Protection Effect Of Toxoplasma Gondii D-3-phosphoglycerate Dehydrogenase

Toxoplasma gondii（T.gondii）is a specialized intracellular parasite that parasitizes nucleated cells and can cause toxoplasmosis,a serious threat to human and animal health.T.gondii in addition to the serious problem of harming human health,will seriously affect the status of the livestock and poultry industry,causing serious economic losses.Cats are the final hosts of T.gondii and are important sources of infection.Mammals and birds can be used as intermediate hosts for T.gondii.T.gondii has serious damage to immunocompromised persons and pregnant animals.T.gondii can rely on the sialic acid receptor on the host cell to invade the host cell.Therefore,in this experiment,the TGME49₂39820 gene named D-3-phosphoglycerate dehydrogenase was screened by the proteomic analysis of previous toxoplasmosis combined with sialic acid.The protein is further cloned and expressed in its gene,the protein is purified,polyclonal antibody is prepared,and IgG is purified.Indirect immunofluorescence and cell adhesion assays confirmed that the protein was unknown on the T.gondii and could bind to cell surface receptors,thereby verifying that it can bind to sialic acid,demonstrating that the receptor bound to the cell surface is sialic acid.The in vitro antibody-inhibitory experiments showed that the protein had a certain ability to inhibit the invasion of the body in vitro.After the mouse was observed for the death time after immunization,it was found that there was no obvious protective effect in vivo.