| Toxoplasma gondii is an important zoonosis,which brings huge disease burden to human beings.Studies have shown that cats and infected pork are common risk factors for toxoplasmosis.Based on the epidemiological investigation of Toxoplasma gondii in pigs and cats,this study also studied the correlation between the existence of cats in pig farms and Toxoplasma gondii infection in pigs.Finally,through the construction of a high degree of biosafety system to prevent and control Toxoplasma gondii in pigs,it provided a reference for the prevention and control of Toxoplasma gondii in pig farms.1: Serological investigation of swine toxoplasma infection in Zhejiang and Hebei provinceTo investigate the natural infection of swine toxoplasma from large-scale pig farms in zhejiang province and hebei province,463 pig serum samples were randomly collected from 23 large-scale pig farms.The results showed that the total infection rate was44.49%.The swine infection rate in zhejiang province was 36.82%,and that in hebei province was 50.38%.According to the analysis of different parities infection,the infection rates of sows with 1-6 parities and high parities(more than 6 secondary)were 42.10%,52.94%,47.17%,45.95%,54.00%,63.64% and 37.50%,respectively,and the total infection rate of sows was 49.28%.The results showed that the infection of swine toxoplasma was very common,and there was a certain degree of infection in different pig farms,but the infection rate in sows was higher.2: Serological investigation of Toxoplasma gondii in cats under different living conditionsIn order to understand the difference of Toxoplasma gondii infection rate in cats living in different environments,228 serum samples of wild cats,urban stray cats and urban pet cats were collected from pig farms in Zhejiang Province from 2018 to 2020.ELISA method was used to test the serum Ig G antibody of Toxoplasma gondii,and the results were statistically analyzed.The results showed that: the positive rates of Toxoplasma antibody in wild cats,urban stray cats and urban pet cats were 64.15%,37.50% and 18.90%respectively,and the average positive rate was 33.33%;the positive rates of Toxoplasma antibody in 2018,2019 and 2020 were 30.93%,39.02% and 28.57%,respectively;the positive rates of Toxoplasma antibody in pig farms were different,ranging from 0% to100% And so on.Conclusion: the infection rate of Toxoplasma gondii in cats is different from that in living environment.Wild cats in pig farms have the highest infection rate,followed by stray cats in cities,and pet cats in cities have the lowest infection rate.3: The correlation between Toxoplasma gondii infection in sows and the presence of cats in pig farmsIn order to understand the correlation between the prevalence of Toxoplasma gondii and the presence of cats and the infection rate of Toxoplasma gondii in large-scale pig farms,three pig farms with large number of cats and high infection rate of Toxoplasma gondii and two pig farms without cats were selected to detect Toxoplasma gondii in cat feces and soil,and detect the serum antibody of Toxoplasma gondii in sows,Then correlation analysis was carried out.Results:(1)the positive rates of Toxoplasma gondii were 40.06%,60.00% and 33.33%,respectively,with an average positive rate of 44.64%;(2)The total positive rates of Toxoplasma gondii in soil were 33.33%,40.00% and 20.00%,respectively,with an average positive rate of 31.11%,while those in soil were 6.67% and20.00%,with an average positive rate of 13.33%.(3)The results of serum Toxoplasma antibody test showed that the average positive rate of pig farms with cats was 61.74%,which was significantly higher than that of pig farms without cats(25.00%),especially for high parity sows,the positive rate could reach almost 100%.Conclusion: the infection rate of Toxoplasma gondii in sows is higher in pig farms with higher infection rate of Toxoplasma gondii.The presence of cats is an important risk factor for Toxoplasma infection in sows.4 : High standard biosafety system for prevention and control of Toxoplasma gondiiIn order to prevent and control Toxoplasma gondii,a high standard biosafety was established in a large-scale pig farm by improving the external biosafety of the pig farm,removing other animals in the farm,and hardening the ground in the production area.Then,the effect of high standard biosafety on the prevention and control of Toxoplasma gondii was evaluated through the detection of serum antibody of sows and the pathogen of Toxoplasma placenta.Results:(1)before the implementation of high standard biosafety,the positive rate of serum in sows was generally high.The positive rates of reserve pigs,pigs with 1-2 parity,pigs with 3-5 parity and sows with more than 6 parity were 50.00%,60.00%,100.00% and 100.00% respectively,with an average positive rate of 79.17%.However,after one and a half years of implementation of high standard biosafety system,the positive rates of serum Toxoplasma gondii in sows above were 16.67% and 16.67%respectively,33.33% and 50.00%,with an average of 29.17%.(2)Before the implementation of the high standard of biosafety,a total of 10 sows with a total of 30 fetal membranes,a total of 5 positive samples were detected,with a positive rate of 16.67%.The five positive samples were from 2 sows,with a positive rate of 20%.However,after the implementation of the high standard of biosafety,no positive samples were detected in 30 fetal membranes of 10 sows,and the positive rates of sows and fetal membranes were 0.Conclusion: the construction of high standard biosafety can effectively prevent and control the transmission and infection of Toxoplasma gondii in pig farms,and provide reference for the prevention and control of Toxoplasma gondii in large-scale pig farms in the future. |
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