Study On The Tissue Culture Of Hippeastrum Hybridum

What is known as the Hippeastrum,generally refers to the general Hippeastrum,which refers to all the plants of the Hippeastrum.In addition to its native species,it also includes a wide variety of native hybrid species.The main species cultivated today are the Hippeastrum varieties bred by foreign hybrids,so they are also known as Hippeastrumhybridum.In recent years,China has imported many new varieties of Hippeastrumhybridum from abroad,Especially the double petals are extremely valuable,So deeply loved by people.But because of its high price,it limits its popularity.Besides the low natural propagation rate and most of the varieties are not able to breed bulblet.Thus,it’s the key to improve the Hippeastrum bulbs quality via establishing an high quality and efficient bulb reproduction technical system.Culture in vitro technique is widely used in production of superior materials in large scale.However,from present research results,including Netherlands and other countries which have developed flower industry,it is short of culture in vitro technique of Hippeastrum,the induction rate of various explants was at an extreme low level,even zero.Traditional scaling propagation of Hippeastrum bulbs is widely used at present,resulting in high frequency of virus.In this paper,the rapid breeding technology by using Hippeastrum hibridum’s horticultural crossbreeding deep red double varieties?s bulb and the aseptic seedling as material were studied,which objective is to lay the foundation for establishing efficient regeneration technical system of Hippeastrum cultured in vitro.The main results were as follows:1.When useing the 2 cm diam Hippeastrumbulbas explant,the suitable induced part is the scales with 6-BAse bulbs.After induction on the original medium for about 3 days,the the bulb piece began to start.After incu6-BAtion for 25 days,initiation rate kept stable.The suitable start medium was MS+4mg/L 6-BA+2mg/L NAA+1g/L AC,and the pH 5.8,Sucrose concentration was 45g/L.Put the start?s bulb slices on subculture medium continues to incubation,when concentration of the 6-BAwas 2 mg/L,as the concentration of NAA decreased,6-BA/NAA ratio increases,the culture medium with 6-BA scales of adventitious bud induction rate was also gradually rise,the suitable proliferation medium was MS+0.2 mg/L NAA+2 mg/L 6-BA.2.When usingthe small bulb of the medium seeded by the culture medium is about 0.5 cm in diameteras explant.the aseptic bulb could be re-cut to induce more offspring,however,the leaves can only induce snow-like callus,and then in turn yellow and withered death.The suitable medium for shoot induce was MS+4mg/L 6-BA+0.5mg/L NAA,p H6.6,Sucrose concentration was 15g/L.The suitable medium for root induc ing was MS+0.5mg/L 6-BA +0.25mg/L NAA,p H5.8,Sucrose concentration was 15g/L.