Screening Of Drought Resistant SSSLs From O. Glumaepatula And Transcriptome Analysisin Rice Germination Stage

In recent years,drought has gradually become a prominent problem affecting China's agricultural production.In order to alleviate the problem of water shortage in rice production and the degree of drought damage of rice,it is considered that the new rice varieties with drought resistance and high yield are cultivated by rice dry cultivation techniques.Improve the drought resistance of rice the most effective and most reasonable way.Exploring drought resistance germplasm resources and clarifying its drought resistance mechanism will contribute to the cultivation of drought resistant varieties.In this study,134 cultivars of wild rice(Oryza rufipogon Griff.)Were used to study the germination of 134 single cultivars(SSSLs)under PEG-6000 simulated drought stress.The drought resistance of HJX74 was significantly higher than that of Japonica indica 74(HJX74).Based on this,the physiological and metabolic changes of SSSLs under drought stress and the regulation of gene expression were analyzed.The main results are as follows:(1)The drought-resistant identification of134 single segment substitution lines(O.glumaepatula)was studied by in-house PEG-6000 simulated drought test in pre-treatment.After screening and re-screening,there are13 SSSLs drought resistance significantly improved(P?0.01)than HJX74 and 9 SSSLs(P?0.01)with drought tolerance were significantly more sensitive than HJX74.Among them,B32 had the most significant drought tolerance than HJX74.(2)Seven cultivars with the same drought resistance were selected,and the drought-resistant materials were 4,and the drought-resistant materials were 2,and HJX74 was used as the control.Found that after PEG stress,the activity of amylase in the seeds and the contents of proline and root soluble protein in the roots and shoots were higher than those in the control.The activity of amylase and the content of soluble protein were positively correlated with the drought resistance of the material.The physiological indexes of drought resistance and the content of proline were not directly related to the drought resistance of the material,and it was not suitable as the physiological index to identify the drought resistance of rice.(3)The root and shoots of B32 and HJX74 were compared with those of HJX74,and the genes were up-regulated and down-regulated by bioinformatics analysis.The expression of differentially expressed genes In more than 2 times the gene 16411,which up-regulated expression of 10579 genes,down-regulated expression of 5832 genes.Among these differential genes,74 genes were differentially expressed in B32 and HJX74 roots,and 169 genes were differentially expressed in B32 and HJX74 buds.There were 46 genes at B32 and HJX74 at different treatment time points Buds are different expression.Twenty-five of these genes were noted in the GO function database,and two genes were annotated in the KEGG database.