Effect Of Catalase Enzyme On Growth Performance,Intestinal Morphology,and Antioxidant Property Of Weaned Piglets

Abstract:This experiment was conducted to evaluate the effects of catalase enzyme（CAT）on growth performance,intestinal morphology,and antioxidant capcity of weaned piglets.240 three-line crossbred（Duroc×Landrace×Yorkshire）weaned piglets were randomly divided into 5 groups,with each group represented by 4 replicates of 12 piglets per replicate.Test group A was fed a basal diet as control group,test group B was fed the basal diet supplemented with 2 500 mg/kg ZnO,while the next 3 experimental groups（C、D、E）were fed basal diet supplemented with 1000、1500、2000 mg/kg CAT,respectively.The trial lasted for14 days.The results showed as follows:1.Compared with the control group,the feed/gain（F/G）of groups D and E were significantly respectively decreased by 3.2%、7.2%（P<0.05）,but the other growth performaces were not significantly different among all groups.2.Compared with the control group,the villus height in ileum of groups D were significantly increased（P<0.05）,and the crypt depth in ileum in group B and D were significantly decreased than those in other groups（P<0.05）.There is no significantly difference in villus height、crypt depth and villus height to crypt depth ratio（V/C）in duodenum、jejunum and ileum among other groups.3.Compared with the control group,malondialdehyde（MDA）content in serum of groups D and E were significantly decreased by 43.7%and 44.7%（P<0.05）,and glutathione（GSH）content in serum of groups B、C、D and E were significantly increased（P<0.05）;but the MDA、GSH and Superoxide dismutase（SOD）content in liver were not significantly different among all groups（P>0.05）.4.Compared with the control group,there is no significantly difference in inhibition ability of superoxide anion and hydroxyl free radical in serum among all groups（P>0.05）,except the inhibition ability of superoxide anion of groups E were significantly increased（P<0.05）.5.There is no significantly difference in the amount of Lactobacillus、Salmonella、Escherichia and total bacteria in feces of ileum ang cecum among all groups（P>0.05）.6.Compared with the control group,the apparent digestibility of ether extract of C、D、E group was significantly increased by 32.62%、33.49%、29.69%（P<0.05）.7.Compared with the control group,after 1h incubation,0.5、1、2、4 mmol/L H₂O₂significantly decrease the cell viability of porcine intestinal epithelium by 34.97%、40.2%、49.36%and 66.81%（P<0.05）,respectively;Compared with the 1 mmol/L H₂O₂ treatment,the cell viability was increased by 30.87%、39.18%and 40.85%（P<0.05）after pretreatment with 100U/mL、400U/m L、1000U/m L CAT for 4h respectively.In summary,this results indicate that the dietary supplemented with 75 U/kg CAT can decrease feed/gain（F/G）and diarrhea rate,while improve intestinal mucosal morphology,antioxidant property and Nutrient Apparent Digestibility.To some degree,dietary supplemented with CAT is comparable to the effect of the dietary supplemented with zinc oxide.