Studies On Genes Related To Meat Traits And Utilization Of Hybrid In Qianbei Brown Goat

Qianbei Brown Goat was a kind of mutton with short hair and skin with an excellent local goat breed in Guizhou.It has the characteristics such as strong adaptability,rough feeding,good meat performance,light odor,good meat quality and good quality of the skin.It is a good material for hybrid utilization and cultivation of new varieties.The Qianbei Brown goat was treated as test subject,Study on the meat quality before and after hybridization between Qianbei Brown Goat and Boer Goat,Nanjiang Yellow Goat and Nubian Goat.Myf5 and Myf6 were candidate genes in this study as influencing meat quality traits.The polymorphism of Myf5 and Myf6 gene in Qianbei Brown Goat was analyzed by DNA mixed pool method and directly sequencing.The expression of Myf5 and Myf6 gene in different tissues of Qianbei Brown Goat were detected by qPCR techniques.Furthermore,the correlation analysis was carried out on the meat quality of Qianbei Brown Goat.Objective to explore the effect of Myf5 and Myf6 gene expression on the characteristics of mutton in Qianbei Brown Goat.In order to provide theoretical basis for breeding of good meat quality Qianbei Brown Goat and also provide theoretical basis for development and utilization.The main results of this study are as follows:1.The meat performance measurement of Qianbei Brown GoatQianbei Brown Goat and Boer Goat hybrid later generation(Boer and QianF1)in the slaughtering performance indexes such as live weight,carcass weightand eye muscle area,etc..and in the meat quality indexes crude protein and crude fat,etc..were compared,Qianbei Brown Goat was improved to some extent.and Qianbei Brown Goat and NanjiangYellow Goat(Nan and QianF1)in the slaughtering performance indexes such as carcass weight,pure meat weightand eye muscle area,etc..and in the meat quality indexes crude protein and water loss rate,etc..were compared,Qianbei Brown Goat was improved to some extent.Qianbei Brown Goat and Nubian Goat(Nubian and QianF1)in the slaughtering performance indexes such as live weight and eye muscle area,and so on.were compared,Qianbei Brown Goat was improved to some extent.The proportion of amino acid content in Boer and QianF1(81.04±2.250),Nan and Qian F1(77.679±2.31)and Nubian and QianF1(81.04±2.250)were balanced in amino acid index,but it was slightly lower than that of purebred Qianbei Brown Goat(82.7735±3.4845).The contents of unsaturated fatty acids and saturated fatty acids were slightly higher than those of pure Qianbei Brown Goat.In different tissues(longissimus dorsal muscle,triceps arm muscle and biceps flexor muscle),the muscle fiber diameter of Nan and QianF1 and Nubian and QianF1 were slightly thicker than that of Qianbei Brown Goat,and the density of muscle fiber was very close.The best effect of hybrid improvement in Boer and QianF1 in the hybridization.2.Detection of Polymorphism of Myf5 and Myf6 Gene and its Association with Meat quality Index.A SNP locus was found at the exon-1 A270 C of Myf5 gene in Black Goat and Qianbei Brown Goat from Guizhou,but no polymorphic loci was detected in Myf6 gene.The correlation analysis between different genotypes of Myf5 gene polymorphism loci with meat quality traits was carried out.It was found that there was a significant difference between different genotypes in the meat quality indexes of Qianbei Brown Goat.3.The expression of Myf5 and Myf6 gene in the related tissue had been researched.The expression of Myf5 gene was detected in heart,liver,spleen,lung,kidney,longissimus dorsal muscle,biceps flexor muscle and triceps arm muscle of Qianbei Brown Goat.The expressions of Myf5 were detected in those tissues,and the expressions in different tissues were different.The order from high to low of expressions were triceps arm muscle,biceps flexor muscle,longest dorsal muscle,kidney,liver,heart,spleen and lung.The expression of Myf5 mRNA in three muscle tissues was significantly higher than that of other tissues(P < 0.01).For Myf6 gene,mRNA expression of Myf6 gene was detected in eight tissues of Qianbei Brown Goat,the order from high to low of expressions were triceps arm muscle,biceps flexor muscle,longest dorsal muscle,liver,heart,kidney,lung and spleen.The expression level in three muscle tissues was significantly higher than that in other tissues(P < 0.01).