Establishment And Application Of Diagnostic Methods For Chlamydia Disease In Parrots

Chlamydia psittaci(Cps)is a member of genus Chlamydia,Chlamydiae,an obligate intracellular microorganism that infects many animals,including humans.The known host includes 15 species of mammals and 130 species of birds.Of these 130 species of birds,57 are of the family Parrotidae.In large rare bird farms,there are many species and quantities of parrots,usually live with more kinds other animal,and the husbandry and operation is very complicated.Thus the diagnosis,prevention and control of Cps of parrots is a problem.This study aimed at the parrot case analysis in the diagnosis of a rare bird field,established the chlamydia psittacosis disease diagnosis method and applies the method of PCR in the populations of disease control and prevention,the main contents are as follows:1.Clinical diagnosis of chlamydia psittaci Eclectust of death,the autopsy,cases of autopsy results show:The bronchi are filled with blood;the spleen is necrotic,swollen,and bleeding.Tissue cytological examination,liver,spleen,air sac contact,Diff-quick staining showed that macrophages in the plasma of a large number of basophilic granules.A purplish red uniform granular chlamydia inclusion was found in the liver and spleen.Pathological examination showed that neutrophil infiltration was the main necrotic area in liver tissue.See the size of poppy seeds purple-blue uniform granular-plasma.Initial diagnosis is that infection of chlamydia psittaci.2.The establishment of the PCR diagnostic method for the chlamydia psittaci In the study,primers were designed based on the main bird outer membrane MOMP gene sequence published on the NCBI.Samples were taken from the above cases and the results showed that two suspected cases were positive and the others were negative.This is consistent with the clinical diagnosis results.By optimizing the conditions of the PCR.APCR detection method for Cps was preliminarily established.Through experimental animal regression test,PCR detection was performed on the embryo of death,and the results showed that the embryoid was infected with chlamydia psittaci.This indicates that the above death case were infected with chlamydia psittaci.3.Application of PCR diagnosis method for psittacin In this study,all the parrots were tested using the Avian Cps antibody test kit,Producted by Biogal in Germany.Positive and negative birds were housed separately in two districts,divided into red and green districts,and 5 species were selected in each districts.4 breeding pairs of birds were selected in each species,including Green-winged Macaws,Blue-and-yellow Macaws,Scarlet Macaws,Eclectic parrots,and orange-winged Amazon.A total of 80 birds were tested.The situation was analyzed and the incidence of offspring in the past two years was analyzed.At the same time,PCR was used to screen parrots in the two districts.The results showed that the incidence of birds in the red district was higher than that in the green district.The positive rate of Cps antibody in the red district was higher than that in the birds of the green district.The incidence of offspring in the red district is higher than that of birds in the green district.Drug treatment for the parrots,By contrast,the results showed that the mortality rate of drinking water was 20%,and the cure rate was 40%.The mortality rate of oral administration was10%,the cure rate was 60%.Intramuscular injection has a 20%mortality rate and 20% cure rate.The total number of white blood cells decreased significantly.It was shown that the oral administration of doxycycline or doxycycline was better than that of drinking water,and the death rate of intramuscular injection was higher.The above results show that the early diagnosis of the animals infected with the Cps and the early treatment with appropriate drugs,separated Positive and negative birds,These are effective prevention and control scheme.These methods could provide a basis for the clinical diagnosis of parrots infected with Cps,which is conducive to the prevention and control of zoological animal diseases and the purification of parrot breeding populations.