Research On Toxicity Of ?-zearalenol To Human Umbilical Vein Endothelial Cells

Zearalenone,an estrogenic activities mycotoxin,is one of the most common mycotoxin contaminants of food and animal feed.The?-zearalenol??-ZOL?is the major metabolites of zearalenone in vivo.Zen and?-ZOL has wide range toxic effect on human and animals health.In this study,human umbilical vein endothelial cells?HUVEC?were used to investigate the cytotoxity of?-zearalenol in vitro.The goal of our research was to evaluate the cytotoxity of?-ZOL?the major metabolites of ZEN?,and elucidate the mechanism of zearalenone toxicity,which could provide the evidences for prevent from zearalenone intoxication.The effects of?-ZOL on HUVEC cell morphological and quantitative traits,cell viability,cell cycle,oxidative stress,cell apoptosis and the cell cycle and apoptosis-related genes expression were detected by using the fluorescence microscopy observation,flow cytometry,qRT-PCR,and the results were as follows:?1??-ZOL treatment could significantly change cell morphology,reduce cell number,decrease cell viability,generate cytotoxity,inhibit cell proliferation,and induce G₂/M phase arrest in HUVEC cells.?2??-ZOL treatment could increase ROS levels,increase the content of MDA,decrease SOD gene expression,induce oxidative stress in HUVEC cells.?3??-ZOL treatment could also induce nuclear pyknotic change the expression of apoptosis-related genes?Caspase3,Caspase6,Bax,Bcl-2?,cause the apoptosis and necrosis in HUVEC cells.Conclutions:The toxic effects of?-zearalenol??-ZOL?on HUVEC cells are indicated by reduction cell viability,inhibition of cell proliferation,arrest cell cycle in G₂/M phase,induction oxidative stress,lead to cell apoptosis.