The Role Of AtbZIP2 In Elicitor-triggered Plant Immunity In Arabidopsis Thaliana

The innate immunity of plants originates from the constant infestation of pathogens,and the interaction mechanism between pathogens and plants is an important part of studying plant immunity.The traditional concept is that stomata is a passive pathway for pathogens to infect plants.However,a new study found that stomata can actively shut down when infecting the interstitial microorganisms to limit the invasion of pathogens.The stomatal immunity is part of the plant's innate immunity.Elicitors can induce plants to produce an immune response that leads to the accumulation of reactive oxygen species and stomatal closure,resulting in a systemic resistance to pathogenic bacteria.Transcription factors are involved in the regulation of plant drought tolerance and disease resistance by regulating stomatal movement.This study explored the function of AtbZIP2 transcription factor from the perspective of elicitors' induction of stomatal and apoplastic defense responses in Arabidopsis thaliana.According to the bioinformatics prediction analysis,the AtbZIP2 expression in the guard cells was high and specific,and was verified by the tissue localization.The phylogenetic analysis of this protein may be related to plant disease resistance.Subsequently,bzip2 homozygous mutants were obtained by three primers and qPCR screening.AtbZIP2 was found to be involved in drought tolerance of Arabidopsis thaliana after drought treatment.Further exogenous stimulation found that AtbZIP2 is involved in Flg22-induced stomatal closure,which may be related to the production of ROS and NO in guard cells.AtbZIP2 was involved in Flg22-induced resistance to Pseudomonas syringae pv.tomatoDC3000(PstDC3000)in stomata and apoplasts through spray and injecting bacteria,Simultaneous inoculation with Sclerotinia sclerotiorum found that AtbZIP2 is also involved in Flg22-induced resistance in Arabidopsis thaliana.These resistance may be related to the accumulation of callose.In conclusion,this study found that AtbZIP2 transcription factor is involved in the defense response of Arabidopsis induced by Flg22,providing a theoretical basis for the molecular mechanism of elicitor-induced plant defense responses.