| In this study,new rice varieties(lines)with different salt tolerance and salt tolerance were used as the materials for reference.The salt and relative salt tolerance methods of Liaoning saline alkali land use institute were used for reference,of which the renowned rice variety 9311 was used as a control,all treated with NaCl solution of 0(CK),0.4%,0.6%,0.8%,and 1.0%concentrations in the bud stage of rice.At the seedling stage,the rice variety with one leaf and one bud cultivated at a concentration of water was made to record its salt-tolerance physiological indicators when placed in NaCl hydroponic nutrient solutions(no soil)with concentrations of 0(CK),0.4%,0.6%,0.8%,and 1.0%.By observing the indicators of bud stage and seedling stage,The genetic diversity of 26 new rice germplasms with different salt tolerance was analyzed by observing the germination period and seedling index and combining with the actual planting situation.The study aimed to ultimately provide basis and evidence for the selection and breeding of salt-tolerant rice from the perspective of morphology,physiology and gene through cluster analysis of SRAP molecular markers.In bud stage experiment under different concentrations of NaCl stress,the results showed that the increase of salt concentration had gradient-inhibiting effect on the germination of rice.The morphological indexes were significantly correlated with the increase of salt concentration,and the salt tolerance of various varieties(lines)had obvious differences.14-14,14-57 and w01 showed strong salt tolerance at high salt concentration(salt concentration of 0.8%and 1%).In seedling stage experiment,indices such as height of seedlings,Fresh weight of the upper ground and leaf chlorophyll contents were mainly recorded.Among them,there was a very significant positive correlation between seedling height,fresh ground weight,root dry weight and chlorophyll content,and the seedling height,fresh weight,root dry weight and chlorophyll content of most varieties decreased with the increase of salt concentration.The salt tolerance of different varieties(lines)was significantly different.14-15,0.8%salt concentration was a strong salt tolerant variety,and 14-12 and 14-30 also had salt tolerance.Combined with the data of bud stage and seedling stage,there showed inconsistent performance of rice with difference strains in both stages,suggesting insignificant correlation between them,namely,salt tolerance ability of rice has varied performance at different stages.Clustering experiment in this study refers to genetic diversity analysis of 26 rice germplasm materials by using SRAP molecular marker technology.9 pairs of primers with good repeatability and abundant polymorphism were screened out from 96 pairs of SRAP primer combinations for PCR analysis,during which a total of 109 bands were amplified.The average number of bands amplified by each pair of primer was 12.1.Among them,the number of polymorphic bands was 80 and the ratio of polymorphic site was 73.39%,suggesting relatively high detection efficiency of SRAP molecular markers for the polymorphism of rice germplasm materials.According to the results of molecular markers,genetic similarity(GS)among all varieties was obtained by adopting NTSYS-pc2.10e software analysis.Then the GS values were used to conduct cluster analysis on test materials by applying UPGMA.The results showed that the genetic similarity coefficients among the 26 tested materials ranged from 0.505 to 0.853,and four major groups could be classified when the genetic similarity coefficient was 0.700.Hereinto,50190 and 9311 had the closest genetic relations,while 14-6 and 15-7 showed the largest genetic differences.Molecular fingerprints of 26 rice strains were constructed by screening out maps with good polymorphism,clearness and high discrimination efficiency from polymorphic primer combinations. |
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