Study On The Mechanism Of Resisting Disease And Promoting Of Arabidopsis Thaliana By Biocontrol Trichoderma

Biocontrol is the world recognized plant disease green prevention method,Trichoderma spp.is a biocontrol agent in the high efficiency biocontrol fungi,and market application dosage form the highest share,Trichoderma asperellum is the dominant strain of Trichoderma spp.Fusarium oxysporum is a stubborn soil-borne disease,difficult to prevent and cure,it is difficult to eradicate,the pathogen harms plant root,causes vascular bundle disease,causes the plant to wither,and in the whole growth period of the plant can occur.Therefore,to improve the mechanism of plant resistance to pathogenic fungi,to explore the mechanism of disease prevention,that is,the mechanism of pathogenic fungi,plant and pathogen,is of great significance for the efficient use of the subsequent Trichoderma spp.preparation.Firstly,the isolation and identification of Trichoderma spp.were carried out,and 1 strains of T.asperellum.were isolated and identified,the colony morphology is consistent with the morphology of the fungal fungi,and the stem of the spore stalk is dispersed,the basic group has 3 bottle stalks,and the bottle stalk is clustered into a dense head-mounted structure,close to the broom-shaped,and the its sequence and the TEF sequence are compared,The result is consistent with morphology.The confrontation test showed that the growth rate of T.asperellum was faster than that of F.oxysporum,the diameter of T.asperellum was twice times of the pathogen diameter,the difference was significant,the inhibitory rate of Trichoderma spp.on F.oxysporum was 66.7%,the competition inhibition was obvious,the pathogen could not grow normally,and the T.asperellum make the pathogen colonies gradually changed to brown.By pathogenicity test of F.oxysporum,the best treatment concentration of pathogen was 5mL/strain,and the growth index of Arabidopsis thaliana seedling was decreased with the increase of toxin concentration,and the difference was significant.The defensive enzymes and soluble proteins of T.asperellum.Tas+ Fox Group and Trichoderma Tas group were higher than those of F.oxysporum(Fox).In the detection of MDA and proline content,both Tas+ Fox group and Tas were lower than group Fox,and the difference was significant.A.thaliana defense Gene PDFl.2B,PDFl.2C RT-qPCR detection,Tas+fox Group and Fox group,the increase of PRI expression is 0.16 times and 1.67 times,the difference is significant,15d,Tas+fox Group and Fox group,compared with the 7d,but the overall trend of increase in the Tas+fox group is 3.29 times,the difference is extremely significant,p<0.001.The study on the resistance to A.thaliana by T.asperellum has proved that T.asperellum has improved the disease resistance of A.thaliana.In the study of the promoting function of Trichoderma spp.,the highest growth index was 60%A.thaliana plants,fresh weight 0.0018g,root length 2.71cm,and aboveground part of the highest fresh weight of 40%of A.thaliana plants,0.0453g,compared with the control,the difference is significant;The activity of pod enzyme with highest 60%was 23.75?/mg prot,the difference was very significant,MDA content decreased significantly with the increase of T.asperellum concentration,which was 0.228?mol/g,and the difference was very significant.The content of Proline was 100%,the content was 23.125,the difference was very significant,60%cell membrane permeability was the lowest,and 6 treatments were not dyed in the active oxygen test.The results showed that the concentration of bacterial fertilizer was the most suitable to promote the concentration.Finally,all the data obtained in this experiment are analyzed statistically.The secondary metabolites of T.asperellum have improved the growth index of A.thaliana seedlings,and the difference is very significant,in summary,the production of Trichoderma spp.is not only the living broth has the effect of promoting and improving the resistance of plants,but also the secretion of small molecule compounds and protein substances can promote A.thaliana growth and development.Through the experiment of T.asperellum isolates in this study,a random sample of soil samples was randomLy extracted from the soil samples from the Gongliu County wild Apple base in Xinjiang Region,and a T.asperellum was successfully isolated.This experiment proves that T.asperellum fertilizer can effectively improve the disease resistance of A.thaliana,and improve the resistance of A.thaliana by increasing physiological and biochermical indices of A.thaliana and increasing the expression level of defensive genes in A.thaliana.The current wild apple disease is serious,the dead apple trees continue to grow,in order to find out the strong strains by T.asperellum isolates,to fertilize the wild apple base,can not only improve the disease resistance of wild apple trees,but also to promote the growth and development of wild apple plants,and then protect the Xinjiang Gongliu County Wild apple Base healthy ecology.