| Soybean lipoxygenase(LOX)belongs to oxidoreductase.Usually,lipoxygenase in soybeans is soluble.Soybean lipoxygenase is a protein containing non-heme iron,Enzyme protein consists of a single peptide chain,it is a single polypeptide protein,it has high content in mature soybean seeds,about 1% to 2% of seeds.The production of flavor and aroma components by lipoxygenase is required by many foods.The development of soybean lipoxygenase can increase the added value of deep processing of soybean and realize the comprehensive processing and utilization of soybean,so,it is of great significance to its research.In this study,we mainly research the following four issues: 70 soybean varieties were tested to determine the Lipoxygenase activity and resource evaluation;difference analysis of soybean Lipoxygenase in different years;correlation analysis of Lipoxygenase activity and agronomic traits.The main results of research was as follows.(1)The 70 soybean varieties were tested by variance analysis,The results showed that the activity of the three isoenzymes among the varieties reached 0.05 significant level.The enzyme activity of Lox1 in soybean grain was the highest,followed by Lox3,Lox2 was was lowest,which may be related to the Lox2 content in soybean grains is relatively few.(2)The differences in soybean lipoxygenase between different years were not significant in one environment.The average total enzyme activity in 2016 was 19.3868,and the average total enzyme activity in 2017 was 20.4257,which was 1.05 times that of 2016.The difference between years is not significant.(3)By clustering analysis,the growth period has a certain correlation with the activity of lipoxygenase.High-lipoxygenase active material,the growth period of 95-100 days.The lipoxygenase active material has a growth period of 99-105 days.Low-lipoxygenase active materials,growth period range 115-120 days.(4)There was a positive correlation between lipoxygenase and plant height and grain number per plant. |
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