Cloning,Transcription Characteristics And Function Investigations Of ZmCRY1b In Maize

The maize is one of the most widely grown food and fodder crops,while the environmental factor light is germane to its important agronomically traits such as plant height,flowering time,yield and quality of fruits.Cryptochromes are blue and ultraviolet-A photoreceptors generally existing in animals,plants and microbials,which mainly regulate photomorphogenesis of plants and circadian rhythms of plants and animals.Therefore,the expression pattern analysis of cryptochrome in maize can lay the foundation of research into the role it plays in the photomorphogenesis of maize.The ZmCRY1 b and ZmCRY2 genes were cloned by RT-PCR.The analysis of their proteins' function domains and the phylogenetic analysis of amino acid sequences were carried out through bioinformatics websites and softwares.qRT-PCR was used to analyze the transcription abundances of ZmCRY1 b and ZmCRY2 genes of inbred line B73 in different tissues and in response to different light treatments.The ZmCRY1 b over-expression vector was transferred to Arabidopsis and phenotype of transgenic plant under white and blue light was obtained.We found that the function domains of ZmCRY1 b and ZmCRY2 proteins were consistent with others,namely the PHR and CCE domains of CRY1 and the PHR domain of CRT2.The consistency of amino acid sequences among ZmCRY,TaCRY and OsCRY were high and higher than AtCRY.Meanwhile,ZmCRY1 b and ZmCRY2 genes highly expressed in leaf with 31.3 or 10.4 times higher than themselves in root,respectively.The transcription abundances of the both genes in different continuous light conditions were higher than them in the dark conditions,especially in the far-red and white light conditions.What's more,they could also greatly respond to the light transitions from the dark to other light conditions.However,the transcription abundances of ZmCRY1 b in all light conditions,long-day and short-day conditions were higher than ZmCRY2,indicating that ZmCRY1 b responded to light and light cycle treatments more effectively than ZmCRY2.The ZmCRY1 b over-expression plants presented the inhibition of hypocotyl elongation and the promotion of cotyledon expansion under white and blue light.In conclusion,ZmCRY have the similar function domains with AtCRY,OsCRY and TaCRY,while their amino acid sequences are also highly consistent,which in turn means they have similar functions.Both ZmCRY1 b and ZmCRY2 genes can greatly respond to different light conditions and light cycle treatments,and play an important role in maize photomorphogenesis.Our results also provide a theoretical basis for the research into the function and application to crop improvement of ZmCRY1 b and ZmCRY2.They can also be used in the orientation improvement of impartment agronomic traits in crops.