The Omics Research On Maize (C4) And Poplar (C3) Regulated By Different Light Signals

The switch from skotomorphogenic to photomorphogenic development is a rapid and dramatic process,red and blue light trigger the light receptors and transfer the signal into light signal transduction pathway in plant,and regulated photomorphogenesis development.This study includes two aspects:(1)Proteome and transcriptome analysis of etiolated maize turning green under different light quality treatment and(2)transcriptome analysis of poplar seedlings under white light treatment.To decipher the effects of various wavelengths of light on photomorphogenesis,we illuminated etiolated maize seedlings with blue(430nm),red(660nm),blue-red mixed and white light for 6h,12h,and 24h respectively.Pair-end RNA sequencing and iTRAQ tag with Nano LC-MS/MS were used to explore the transcriptome and proteome of maize etiolated seedlings in different light condition,and also the phenotype and physiological change in photomorphogenesis development were detected.Transcriptome were performed on Illumina HiSeq2000 platform.26,972 genes were identified,and 10,436 genes were differentially expressed in common of the 13 samples.The GO and MapMan enrichment results showed that,after 6h treatment,the genes of photosynthesis and energy metabolism were significantly up-regulated,the cell wall,hormone metabolism,kinase signaling related genes were notable down-regulated.476,217 alternative splicing events,classified into 8 types of alternative splicing,were discovered in transcriptome data.50,467 events were annotated in maize genome and the other 428,750 alternative splicing events were novel.41,188 transcripts were detecting as novel transcripts for annotated loci,and 3,491 were unknown transcripts located in intergenic region,including 876 multi-exonic transcripts.Differential exon usage was analyzed with DEXSeq and 8,792,4,817,3,391,2,842 DEU genes in blue,red,white and blue&red light treatment respectively.The enrichment results of DEU showed that the regulation of DEU with red and blue light is different.Notably,lots of genes in photosynthesis pathway were showed DEU in response to B and/or R.10,867 annotated proteins and 747 unannotated proteins were identified in 6h proteome results.The parallel analysis showed that,blue light is more efficient than red light on improving expression of the proteins in chloroplast and photosynthesis.Strikingly,526 novel protein isoforms have changes in functional domains compared with their most similar isoform.In leaves,auxin contents were rapidly decrease after light treatment.The difference of Pchlide transform to Chlide under different light treatments showed that regulation of POR activity by different light quality is obviously distinct.Chloroplast developed fastest in white light treatment and faster in blue light than red light treatment.The results suggested that blue and red light regulated photosynthesis,cell cycle,carbon metabolism,splice/edit of RNA,auxin synthesis,chlorophyll synthesis pathway as light signal.AS is an important mechanism for increasing protein diversity and acts as a protein function switch allowing rapid light response through the addition or removal of functional domains.The richness of novel transcripts and protein isoforms also demonstrates the potential and importance of integrating proteomics into genome annotation in maize.To research how light regulates 84K poplar(Populus alba × Populus glandulosa)seedlings,the 84K poplar grew in dark for 3 days were treated with red,blue,white and blue plus red lights for 6h and proceed RNA-seq.The treatment time was 6 hours.Transcriptome sequencing analysis.The results showed that there were 2483 genes expressing a total expression,of which 576 genes were up-regulated and 1867 genes were down-regulated.The most significant enriched GO terms were carbohydrate metabolism,secondary metabolism,stress,response to stimulate.585 genes can classify into 33 bins of Mapman category,RNA,secondary metabolism,protein,stress,cell wall,signaling.bHLH,ap2/b3,wrky transcriptional factors families were significant enriched.Protein,amino acid,carbohydrate,nucleotide metabolism prefers to up-regulated.The plant hormones have littles changes besides ethylene,which were impact most under light regulation.The results showed that poplars were transform from dark to light with the proceeding of basic metabolism pathway and fundimatal materials synthesis,inhibited of the elongation and development of cell,defensed ROS induced by light and other stresses.The research used omics technology to analysis the dark-to-light process of maize and poplar,indicated the regulated mechanism,enriched the annotation of maize genome,and provide the theory basic to research of C3 and C4 plants.