Study On Winter Dormancy Of Thesium Chinese And Its Carbohydrate Accumulation

Thesium Chinense Turcz.(Santalaceae)is an obligate root hemiparasite and the whole weed resource is a traditional medicine in China for the treatment of rheumatism and various inflammatory diseases,has endured centuries of exploitation.T.chinense can be found in China,Japan and Korea.In our country T.chinense mainly distributed in jiangsu,anhui,zhejiang,hebei,jiangxi and other provinces.Recently,T.Chinense have have been shown to possess bacteriostatic and anti-inflammatory activities,it is called“plant antibiotics”.Witer dormancy is a important phenomenon of T.Chinense.T.chinense are growing develop by seed in the first year and they are growing up very slow.The root neck bud will grow in autumn and winter,and grow up quickly in the second year.Plant morphology and weight of T.chinense always have a big change before and after dormancy.Winter dormancy has become an important phenomenon during the grouth of T.chinense.In order to explore reasonable artificial cultivation pattern of T.Chinense,the study ues wild T.chinense surrounding Nanjing as experiment meterial.Using fixed-point notation to observe the phenological period of T.chinense.Then research the phenomenon of winter dormancy by using the method of plant physiology and molecular experiments.The results are as follows:1.The normal life cycle of T.chinense is two years..chinense are growing develop by seed in the first year.Seedlings which sprout earlier will die in summer.Others can go through the summer,and then keep in the state of dormancy in winter.T.Chinense are growing by the root neck bud in the second year,and they grow shorter.2.Carbohydrate mainly stored in the root of T.Chinense in October,they are the basis of energy for T.Chinense to grow in the second year.During the process of dormancy,Starch and soluble sugar can mutual transformation in different periods.T chinense have sufficient carbohydrate to maintain growth and also has a lot of small molecules to improve their ability to fight against adversity.3.During the process of dormancy,buds are the most sensitive for external environment.Cold tolerance of buds may be higher than root.The organs underground and above ground always keep in different dormant state during the winter.Buds are formed in October and November,then keep dormant in December,However,dormancy of organs underground always began in October.4.TcPRO and TcCHL both have the function to resist cold.During the winter dormancy,their relative expression levels were generally increases with the deepening of dormancy,and relative expression levels were the highest in January.The function of TcPRO only can be activated by lasting low temperature.TcMAN almost have no expression during the dormancy except in January.TcMAN may be mainly used to produce soluble sugars by degrade starch in phosphorylation pathway.Then provide a lot of small molecules to improve their ability to fight against adversity.